Method for extracting, separating and purifying flax lignans from flax cakes

A technology of flax lignans and flax cakes, which is applied in the fields of chemical instruments and methods, organic chemistry, sugar derivative preparation, etc., can solve the problems of low extraction temperature, harsh conditions, high cost, etc., and achieve simple process operation and low condition requirements High, relatively high-purity effect

Inactive Publication Date: 2012-11-28
上海红马饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its simple and convenient operation, low extraction temperature and good quality, the solvent extraction method is the earliest and most widely used method, and it is easy to produce on a large scale. However, the yield of SECO is limited, and the extraction rate of subcritical water extraction is relatively high, but the conditions are harsh. There are many problems with industrial applications
Enzymatic extraction, high cost, not conducive to large-scale industrial production application

Method used

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  • Method for extracting, separating and purifying flax lignans from flax cakes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) After crushing the dried flax cake, pass it through a 100-mesh sieve, weigh 100g, add an appropriate amount of 70% ethanol solution 500ml, seal and soak for 1.5h, put it in an ultrasonic device, set the power to 300W, and the temperature to room temperature , treated for 20 minutes, then continued to stir and extract, and after 3 hours, centrifuged at 1500rmp for 10 minutes to remove the precipitate and obtain the supernatant;

[0030] (2) Add NaOH solution to the supernatant obtained in the previous step to adjust pH=12, stir and extract for 2.5h, keep pH=12 constant in this process, centrifuge at 3000rmp for 10min, neutralize the supernatant with HCl solution, and adjust the pH to 4.0;

[0031] (3) The crude extract obtained in the previous step is adsorbed through AB-8 type macroporous resin chromatography, and the flow velocity is controlled at 0.5cm / min;

[0032] (4) Elute with 20% ethanol solution to obtain an alcohol solution containing flax lignans, concent...

Embodiment 2

[0034] (1) After crushing the dried flax cake, pass through a 100-mesh sieve, weigh 100g, add an appropriate amount of 60% ethanol solution 800ml, seal and soak for 1 hour, place in an ultrasonic device, set the power to 300W, and the temperature to room temperature, Treat for 30 minutes, then continue to stir and extract. After 3.5 hours, centrifuge at 1500rmp for 5 minutes to remove the precipitate and obtain the supernatant;

[0035] (2) Add NaOH solution to the supernatant obtained in the previous step to adjust pH=12.5, stir and extract for 2h, keep pH=12.5 unchanged in this process, centrifuge at 3000rmp for 10min, neutralize the supernatant with HCl solution, and adjust the pH to 4.2;

[0036] (3) The crude extract obtained in the previous step is adsorbed through AB-8 macroporous resin chromatography, and the flow rate is controlled at 0.5cm / min;

[0037] (4) Elute with 20% ethanol solution to obtain an alcohol solution containing flax lignans, concentrate in a vacuum...

Embodiment 3

[0039] (1) After crushing the dried flax cake, pass it through a 100-mesh sieve, weigh 100g, add an appropriate amount of 50% ethanol solution 1000ml, seal and soak for 0.5h, put it in an ultrasonic device, set the power to 300W, and the temperature to room temperature , treated for 40 minutes, then continued stirring and leaching, and after 4 hours, centrifuged at 1500rmp for 5 minutes to remove the precipitate and obtain the supernatant;

[0040] (2) Add NaOH solution to the supernatant obtained in the previous step to adjust pH=11.5, stir and extract for 1.5h, keep pH=11.5 constant in this process, centrifuge at 3000rmp for 10min, neutralize the supernatant with HCl solution, and adjust the pH to 4.5;

[0041] (3) The crude extract obtained in the previous step is adsorbed through AB-8 macroporous resin chromatography, and the flow rate is controlled at 0.5cm / min;

[0042] (4) Elute with 20% ethanol solution to obtain an alcohol solution containing flax lignans, concentrat...

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Abstract

The invention discloses a method for extracting, separating and purifying flax lignans from flax cakes, which comprises the following steps: pulverizing dry flax cakes, adding an ethanol solution, soaking in a sealed environment for 0.5-1.5 hours, and treating with ultrasonic for 20 minutes; leaching while stirring, and centrifuging to obtain a flax lignan crude extract; regulating the pH value of the flax lignan crude extract to 11.5-12.5, leaching for 1.5-2.5 hours while stirring, centrifuging to remove the precipitate, and regulating the pH value of the supernatant to 4.0-4.5; adsorbing the supernatant by macroporous resin chromatography; eluting with a 20% ethanol solution to obtain an alcohol solution containing flax lignans, concentrating with a rotary evaporimeter, and carrying out freeze-drying to obtain the finished product flax lignans. The invention also discloses flax lignans prepared by the method. The invention has the advantages of simple extraction technique, low production facility request and high extraction efficiency, and is obviously superior to the traditional solvent extraction method.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying flax lignans from flax cake, and belongs to the field of development and application of biologically active substances. Background technique [0002] Flaxseed has become the seventh largest oil crop in the world and the fourth largest oil crop in my country. The main flax planting regions and countries include Canada, India, China, the United States, Argentina, the Nordic region and some Commonwealth of Independent States countries, of which Canada's flaxseed production Highest. The main production areas of flax in China are mainly distributed in North China and Northwest China. Gansu, Inner Mongolia, Ningxia, Hebei and other provinces have the highest flaxseed production, accounting for about 75% of the total national production, followed by Xinjiang, Jilin, Shaanxi and Qinghai, and Southwest China. There are sporadic plantings. In recent years, the production of flaxseed has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 赵肖刘国庆胡卫华钱晓勇
Owner 上海红马饲料有限公司
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