Method and device for measuring or monitoring tissue or cell transmembrane potential changes

Abstract

The invention discloses a method and a device for measuring or monitoring tissue or cell transmembrane potential changes. The method includes: firstly, converting the cell transmembrane potential changes to fluorescence signals, measuring variable quantity deltaF of the fluorescence signal light intensity and changes deltaVm of cell transmembrane potential, then computing optical action potential, and computing to obtain the action potential for each point so that an equal potential pattern at a certain moment can be reconstructed when the action potential of each point in a frame image is computed; and secondly, utilizing a simplified algorithm to obtain an average resting potential fluorescence image, then directly subtracting a background image from the obtained fluorescence image, and computing to obtain the equal potential pattern finally. The method and the device for measuring or monitoring the tissue or cell transmembrane potential changes can meet requirements of most electrophysiological studies. Dyestuff can be poured onto cell membranes by the aid of a peripheral support system, basic stability is maintained within a measured time, and cell viability is maintained during experiment.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a technology that can be used for physiological or pathological cell membrane potential detection. The voltage-sensitive dye is used to convert the membrane potential change signal of cells or tissues into optical information for recording and reconstructing functional images. It is an applied research invented by combining optical technology and image information processing technology in the field of biomedical engineering, especially a method and device for measuring or monitoring tissue or cell transmembrane potential changes. Background technique [0002] The electrical phenomena exhibited by living organisms during life activities are called bioelectricity, and almost all physiological functions are accompanied by some kind of bioelectrical changes. Common detection instruments for these bioelectricity include Near Infrared (NIR), functional Magnetic Resonance Imaging (fM...

AI Technical Summary

Problems solved by technology

In recent years, some foreign published literature such as Tang D, Li Y, Wong J, etal. "Characteristics of a charged-coupled-device-based optical mapping system for the study of cardiac arrhythmias". J Biomed Opt, 2005, 10 (2):1-7. Using laser and xenon lamp as light source, Brown NH, Dobrovolny HM, Gauthier DJ, etal "A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector". Biophys j,2007,93 :254-263 use optical fiber and grating for wavelength selection, the system is more complicated

These have failed to realize the ease of use of the system, and the cost is expensive, which limits the application of this technology

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