New lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape

A technology of betaine aldehyde dehydrogenase and Brassica napus, which is applied in the directions of genetic engineering, plant genetic improvement, oxidoreductase and the like

Inactive Publication Date: 2012-12-12
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

However, the homologous gene of Arabidopsis BADH1 (ALDH10A8) in rapeseed has not been reported

Method used

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  • New lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape
  • New lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape
  • New lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with specific embodiments.

[0010] ① Obtaining sterile seedlings: Soak Brassica napus seeds in 75% ethanol for 1 min and 0.1% mercuric chloride for 8 min to sterilize them, wash them repeatedly with sterilized water for 3 to 5 times, and then place them on MS solid medium; 25°C in an artificial climate chamber, 70% relative humidity, and 16h light / 8h dark conditions for growth.

[0011] ② Extraction of rapeseed total RNA and synthesis of total cDNA: Take rapeseed leaves about 3 weeks after germination, use Trizol kit to extract total RNA; use RNase-free DNase I to remove residual DNA from the obtained total RNA, and then reverse The first-strand cDNA was generated using the transcription kit: Oligo(dT)18 primer was used for reverse transcription, and after incubation at 42 °C for 30 min, the enzyme was inactivated by heating at 85 °C for 5 min. The resulting cDNA was stored at -20°C for subsequent ...

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Abstract

The invention discloses a new lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape. The nucleotide sequence is as shown in SEQ ID NO:1; and the coded amino acid sequence is as shown in SEQ ID NO:2. A new BADH gene sequence is obtained by cloning in cabbage type rape and is named as BnBADH-1. Compared with Arabidopsis thaliana BADH 1 (ALDH10A8), the BnBADH-1 has a homology of 90.24% in nucleic acid sequence and a homology of 93.41% in amino acid sequence. Compared with another rape BADH (AY351634) existing in GeneBank, the BnBADH-1 has a homology of 75.79% in nucleic acid sequence and a homology of 77.93% in amino acid sequence. Thus, the BnBADH-1 is a new gene different from the AY351634.

Description

technical field [0001] The invention particularly relates to a new betaine aldehyde dehydrogenase gene BnBADH-1 in Brassica napus. Background technique [0002] Betaine is an important osmotic regulator in higher plants, which can protect biological macromolecules from denaturation under high electrolyte concentration, maintain cell turgor, do not interfere with cell structure and function, and have The effect of accelerating protein synthesis is beneficial to improving the tolerance of plants to drought stress or salt stress. Betaine aldehyde dehydrogenase (BADH) is an important key enzyme for the synthesis of betaine in higher plants. Brassica napus is one of the most important oil crops in my country. The planting area and total output of rapeseed in my country rank first in the world. Drought stress often seriously affects the yield of rapeseed. Therefore, it is of great theoretical and practical significance to study the BADH gene of olive-shaped rapeseed. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02
Inventor 蒋梁材柴靓李浩杰蒲晓斌张锦芳蒋俊
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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