Method for breeding fast fermentation yellow wine Saccharomyces cerevisiae

A technology for Saccharomyces cerevisiae and rice wine, applied in the field of microbial breeding, can solve the problems of single means, cumbersome process, large blindness, etc., and achieve the effect of improving screening efficiency

Inactive Publication Date: 2012-12-19
JIANGNAN UNIV
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Problems solved by technology

[0004] The main purpose of the present invention is to overcome the shortcomings of single means, large blindness, cumbersome process, heavy screening workload and low efficiency in the breeding of rice wine Saccharomyces cerevisiae at the present stage in my country, and provide a method for screening fast-fermenting excellent rice wine Saccharomyces cerevisiae

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  • Method for breeding fast fermentation yellow wine Saccharomyces cerevisiae
  • Method for breeding fast fermentation yellow wine Saccharomyces cerevisiae
  • Method for breeding fast fermentation yellow wine Saccharomyces cerevisiae

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[0015] The present invention will be further described below in conjunction with specific examples, and specific examples should be understood as illustrations only, rather than limitation, and the protection scope of the present invention cannot be limited by the following examples.

[0016] The technical route of the present invention is: carry out UV mutagenesis to the original rice wine Saccharomyces cerevisiae strain XY (yeast used in the production of a certain rice wine factory in a certain Zhejiang area), and screen for clotrimazole resistance (CTZ r ) yeast, and the CTZ r The yeast is screened in two stages, and the yeast with high fermentation rate is finally screened out. The specific steps to implement this method are as follows:

[0017] (1) Preparation of sugar solution medium: Weigh 500 g of glutinous rice, soak for 3 days, steam, add 1 L of water, 200 μL of glucoamylase, liquefaction enzyme, acid protease, 45 g of wheat koji, and mix well. Store at -20°C unti...

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Abstract

The invention relates to a method for breeding fast fermentation yellow wine Saccharomyces cerevisiae, belonging to the field of microbial breeding. The method comprises the following main steps: performing pretreatment to obtain stable chemical (fungus inhibitor) resistant saccharomycetes, and screening the resistant saccharomyces to obtain the fast fermentation yellow wine Saccharomyces cerevisiae. The method is a method for screening stable chemical-resistant yellow wine Saccharomyces cerevisiae. The method can reduce the blindness brought by common screening work and improve the screening efficiency, and is of a certain guiding significance in the screening of fine saccharomyces, thereby providing important information for the breeding of yellow wine Saccharomyces cerevisiae in China.

Description

technical field [0001] The invention relates to a method for selecting and breeding rice wine Saccharomyces cerevisiae, improving screening efficiency and obtaining rapid fermentation of rice wine Saccharomyces cerevisiae, belonging to the field of microbial breeding. Background technique [0002] Yellow rice wine is a typical representative of my country's national characteristic alcoholic beverages. It is also known as the world's three major ancient wines together with beer and wine. The unique brewing method of wheat koji saccharification and distiller's fermentation makes rice wine brewing technology an outstanding representative of the wisdom of the Chinese nation (qu is known as China's fifth greatest invention). With the development of modern rice wine brewing technology, the traditional operation mode of vat fermentation is gradually replaced by modern and mechanized rice wine production technology represented by large tank fermentation. The clarification of the br...

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Application Information

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IPC IPC(8): C12N1/18C12R1/865
Inventor 徐岩黄笃厚陈双
Owner JIANGNAN UNIV
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