Method for inducing development of natural tetraploid loach male nucleus in cold shock way

A technology of tetraploid loach and androgenesis, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as death, low survival rate of diploid, destruction of cytoplasm and other organelles, and avoid operating procedures , It is conducive to large-scale production and use, and the effect of shortening the breeding time
CN102835338AInactive Publication Date: 2012-12-26DALIAN OCEAN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN OCEAN UNIV
Publication Date
2012-12-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for inducing the development of natural tetraploid loach male nucleus in a cold shock way. The method has the advantages that the ovum inactivation and the chromosome doubling treatment are not needed, the male nucleus development diploid loach can be fast and efficiently produced, and in addition, the hatching rate is high. The method is sequentially implemented according to the following steps that the natural male tetraploid loach and female diploid loach are taken for hastening parturition, the semen of the male tetraploid loach and the ovum of the female diploid loach are respectively taken, the obtained semen is diluted by Kurokura's solution for 100 times for use, the diluted semen is mixed with the ovum to form fertilized ovum, and the fertilized ovum is placed into ice water being 3 DEG C to be soaked for 60 minutes and is then placed into normal temperature water for hatching.
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Description

technical field

[0001] The invention relates to a method for androgenesis of polyploid fish, in particular to a cold shock method for rapidly and efficiently producing androgenetic diploid loach with high hatching rate without egg inactivation and chromosome doubling treatment A method for inducing androgenesis in natural tetraploid loach. Background technique

[0002] Androgenesis refers to the reproductive method of using sperm to produce individuals with only paternal genetic material. Since the genetic material of androgenesis comes entirely from the father, without the participation of female pronuclei, it can be used for the establishment of pure lines, and at the same time, it can freeze the sperm of endangered species, which has special significance for the protection of species. In addition, in aquaculture, because different sexes of many important economic fishes often show different production performances, such as differences in body shape and body color, growth...

Claims

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