Single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof
A single nucleotide polymorphism and milk goat technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial measurement / inspection, etc., can solve the problems of microRNA-431 that have not been reported, and achieve low cost and low cost , high precision effect
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[0036] In the present invention, firstly, primers are designed according to the conserved sequence of the microRNA-431 gene, and the genomic DNA pools of four goat varieties are respectively used as templates to carry out PCR amplification, and the PCR products are purified and sequenced. Then, analyze the sequence diagram and compare the sequences to screen out SNP sites; secondly, perform MluI-RFLP detection of polymorphic sites in the tested population; finally, perform population genetics according to the genotypes detected in the population Statistical analysis and association analysis of lactation volume screened out molecular markers closely related to lactation traits of dairy goats. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.
[0037] a. Cloning of goat microRNA-431 gene DNA sequence and detection of its polymorphism
[0038] 1. Collection and processing of goat blood samples
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