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Method for extracting rosmarinic acid from folia perillae acutae

A technology of rosmarinic acid and perilla leaves, applied in the separation/purification of carboxylate, organic chemistry and other directions, can solve the problems of low product yield and low product purity of rosmarinic acid, and achieve high yield, The effect of low production cost and few steps

Active Publication Date: 2013-01-02
山西金紫苏生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rosmarinic acid product that this method obtains not only purity is lower, and the yield of product is also very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Collect 10 kg of fresh perilla leaves at the early stage of fruiting, wash them with tap water, cut them into strips about 2 cm wide, put them into 60% ethanol aqueous solution with pH=3.0 according to the ratio of material to liquid 1:10 (kg / L), and place Put it into an ultrasonic extractor, extract for 10 minutes at an extraction temperature of 50°C and a power of 125W, collect the extract, and extract the extracted solid once more under the same conditions. Combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, apply AB-8 macroporous resin, wash with pure water to remove impurities, desorb with 65% ethanol aqueous solution to obtain 2L desorption liquid, and obtain crude extract after concentrating under reduced pressure 200 grams, rosmarinic acid content 25%. The crude extract was dissolved in 1 L of acid water with pH=3, extracted twice with n-butanol, and the organic phase was concentrated under reduced pressure to...

Embodiment 2

[0019] Collect 10 kg of perilla leaves in the early stage of fruiting, wash them with tap water, cut them into strips about 2 cm wide, and put them into 70% ethanol aqueous solution with pH=4.0 according to the ratio of solid to liquid 1:10 (kg / L). Put it into an ultrasonic extractor, extract for 10 minutes under the conditions of extraction temperature 60°C and power 175W, collect the extract, and extract the extracted solid twice under the same conditions. Combine the extracts obtained three times, concentrate under reduced pressure until there is no alcohol smell, apply AB-8 macroporous resin, wash with pure water to remove impurities, and desorb with 75% ethanol aqueous solution to obtain 2L of desorption liquid, which is concentrated under reduced pressure to obtain crude extract 220 gram, rosmarinic acid content 28%. The crude extract was dissolved in 1 L of acid water with pH=3, extracted with n-butanol three times, and the organic phase was concentrated under reduced p...

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Abstract

The invention discloses a method for extracting rosmarinic acid from folia perillae acutae. According to the method disclosed by the invention, a rosmarinic acid product with the purity of more than 98% is prepared by carrying out processes such as cleaning, slicing, ultrasonic extraction, macroporous resin absorption, desorption and concentration, acid water dissolution, extraction and column chromatography on silica gel on a raw material folia perillae acutae at the initial stage of seeding. By utilizing the method disclosed by the invention, a high-purity rosmarinic acid product can be obtained; a rosmarinic acid crude product with the purity of 20-30% and a rosmarinic acid crude product with the purity of 60-75% can be obtained during the production process so as to meet different requirements; and the method provided by the invention is short in extraction time, simple in operation, less in steps, low in production cost and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant extract production, and relates to a method for extracting rosmarinic acid, in particular to a method for preparing rosmarinic acid by using perilla leaves as extraction materials. Background technique [0002] Rosmarinc acid (RosA for short) is a phenolic acid compound with various physiological activities, mainly found in Labiatae, Umbelliferae and Boraginaceae plants. Rosmarinic acid is a dimeric derivative of caffeic acid, molecular formula C 18 h 16 o 6 , has various biological activities, including: antioxidant, antibacterial, anti-inflammatory, anti-HIV and anti-platelet aggregation, etc. Rosmarinic acid contains 4 phenolic hydroxyl groups and has strong free radical scavenging activity. Its antioxidant activity is not only stronger than natural antioxidant vitamin E, caffeic acid, folic acid, etc., but also stronger than widely used synthetic antioxidants. 2,6-di-tert-butyl-4-methylpheno...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
Inventor 李会珍李晓君张志军孙子文
Owner 山西金紫苏生物科技股份有限公司
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