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Method for extracting cordycepin and polysaccharide from cordyceps militaris

A technology for cordycepin and cordyceps militaris, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of difficult industrial production, long extraction period, high cost, and achieve high extraction rate, short extraction time, The effect of low energy consumption

Inactive Publication Date: 2013-01-02
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method adopts thermal reflux extraction and macroporous resin separation, and the extraction cycle is long and the temperature is high, resulting in high product yield
Another example is the patent (application number: 200910043666.4) "Continuous Extraction of Cordyceps Volatile Oil, Cordycepin, Cordycepic Acid and Cordyceps Polysaccharide from Cordyceps militaris". Difficult to industrialize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Extraction: The raw material of Cordyceps militaris is crushed into granules, 3 kg is put into a subcritical extraction kettle, and deionized water is introduced into it, and the extract is dynamically extracted at a temperature of 150° C. and a pressure of 6 MPa for 20 minutes to obtain an extract.

[0017] Separation: Concentrate the extract to a density of 1.1, add 6 times the volume of 95% ethanol, stir and precipitate fully, dissolve the precipitate in water again, add 8% trichloroacetic acid to remove the protein, dissolve again, and dry to obtain 61g of Cordyceps polysaccharide , the detected content is 91%. Combine the two alcohol precipitation liquids, concentrate under reduced pressure to no alcohol, add to an activated carbon column for adsorption, elute with 70% ethanol solution with 4 times the column volume, concentrate the eluent under reduced pressure, place crystallization, dry the crystals to obtain cordycepin, and determine the content 94%, yield 85%....

Embodiment 2

[0019] Extraction: The raw material of Cordyceps militaris is crushed into granules, 3 kg is put into a subcritical extraction kettle, and deionized water is introduced into it, and the extract is dynamically extracted at a temperature of 130° C. and a pressure of 3 MPa for 30 minutes to obtain an extract.

[0020] Separation: Concentrate the extract to a density of 1.05, add 5 times the volume of 95% ethanol, stir and precipitate fully, dissolve the precipitate in water again, add 10% trichloroacetic acid to remove protein, dissolve again and precipitate with alcohol, and dry to obtain 68g of Cordyceps polysaccharide , the tested content is 85%. Combine two alcohol precipitation liquids, concentrate under reduced pressure to no alcohol, add to activated carbon column for adsorption, 4 times column volume 80% ethanol solution is eluted, eluate is concentrated under reduced pressure, placed to crystallize, and the crystals are dried to obtain cordycepin, and the content is deter...

Embodiment 3

[0022] Extraction: The raw material of Cordyceps militaris is crushed into granules, 3 kg is put into a subcritical extraction kettle, and deionized water is poured into it, and the extract is dynamically extracted at a temperature of 140° C. and a pressure of 5 MPa for 40 minutes to obtain an extract.

[0023] Separation: Concentrate the extract to a density of 1.1, add 6 times the volume of 95% ethanol, stir and precipitate fully, dissolve the precipitate in water again, add 4% trichloroacetic acid to remove protein, dissolve again and precipitate with alcohol, and dry to obtain 65g of Cordyceps polysaccharide , the detected content is 89%. Combine two alcohol precipitation liquids, concentrate under reduced pressure to no alcohol, add to an activated carbon column for adsorption, elute with 90% ethanol solution for 3 times the column volume, concentrate the eluent under reduced pressure, place crystallization, dry the crystals to obtain cordycepin, and determine the content ...

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Abstract

The invention discloses a method for extracting cordycepin and polysaccharide from cordyceps militaris. The method includes: crushing cordyceps militaris serving as raw materials, filling the crushed cordyceps militaris into a subcritical extraction kettle, feeding deionized water, performing countercurrent extraction for 10-60 minutes at the temperature of 100-200 DEG C and under the pressure of 1-15MPa so that extracting solution is obtained; after the extracting solution is concentrated, adding 5-6 times volume of 95% ethanol, sufficiently stirring for sufficient precipitation, dissolving precipitate by water again, adding trichloroacetic acid with the concentration of 3%-10% to remove protein, performing alcohol precipitation again and drying so that cordyceps polysaccharide is obtained; and combining two-time alcohol precipitate, concentrating to enable the alcohol precipitate to be free from alcohol by pressure reduction, adding into an activated carbon column for adsorption, eluting with ethanol solution, concentrating eluant by pressure reduction, standing for crystallization and drying crystal substances so that the cordycepin is obtained. The method has the advantages of short extraction period, high product yield and simple technological operation and is suitable for industrialized popularization.

Description

Technical field [0001] The present invention is the field of natural activity ingredients, involving a method of extracting cordyceps and polysaccharides from Cordyceps sodermia. Background technique [0002] Cordyceps cordyceps is the type of subcontrapelomais, the pyramidis, and the genus of the genus Cordyceps, and the cordyceps, also known as the northern Cordyceps sinensis, the northern cordyceps, and Cordyceps.Through the identification, it is believed that it is the same genus as Cordyceps sinensis, named pupae.Mainly produced in Yunnan (Kunming, Anning, Jiangchuan), Jilin (Antu, Yongji), Liaoning (Shenyang), Inner Mongolia (Zhelima), born in needles, broad -leaved forests or mixed forests.On the body.It can be cultivated in batches of family silkworms and silkworms, which are similar or even better with pharmacological, pharmacology and wild species. [0003] Modern studies have found that the northern worming sinensis contains a large amount of cordyceps. The special hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV