Preparation method for spirulina antitumor polypeptide

A spirulina, anti-tumor technology, applied in anti-tumor drugs, fermentation, drug combination and other directions, can solve problems such as human injury

Active Publication Date: 2013-01-02
广东省几何细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current treatment methods for cancer patients are mainly chem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of spirulina anti-tumor polypeptide, the steps are as follows:

[0019] (1) Dissolve 25 g of spirulina powder in 250 mL of Milli-Q water, and stir magnetically for 30 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was broken by sonicating 200 times at an ultrasonic power of 130W at intervals of 10 s for 5 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at 4 °C and 7000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the spirulina protein powder.

[0020] (2) Based on the spirulina protein powder obtained in step (1), a spirulina protein solution...

Embodiment 2

[0023] The preparation method of spirulina anti-tumor polypeptide, the steps are as follows:

[0024] (1) Dissolve 30 g of spirulina powder in 200 mL of Milli-Q water, and stir magnetically for 20 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was disrupted by sonicating 200 times at an ultrasonic power of 50 W with intervals of 5 s and 6 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at 4 °C and 6000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the spirulina protein powder.

[0025] (2) Based on the spirulina protein powder obtained in step (1), a spirulina protein solu...

Embodiment 3

[0027] The preparation method of spirulina anti-tumor polypeptide, the steps are as follows:

[0028] (1) Dissolve 30 g of spirulina powder in 400 mL of Milli-Q water, and stir magnetically for 50 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was disrupted by sonicating 120 times at 150 W ultrasonic power at intervals of 10 s for 6 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at a temperature of 4 °C and a speed of 5000 r / min for 40 min to remove the precipitate to obtain a protein supernatant, and then rapidly freeze-dried in a vacuum to obtain Spirulina protein powder.

[0029] (2) Based on the spirulina protein powder obtained in step (1), a spir...

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Abstract

The invention provides a preparation method for spirulina antitumor polypeptide. According to the invention, spirulina protein is extracted mainly by using a process of combined multigelation and ultrasonication, then is hydrolyzed by papain and finally filtered with an ultrafiltration centrifuge tube so as to obtain spirulina polypeptide with a molecular weight in ranges of 0 to 3 KD, 3 to 5 KD, 5 to 10 KD and more than 10 KD. The spirulina polypeptide prepared in the invention has antitumor activity and is beneficial for development and utilization of antitumor health food and medicinal products.

Description

technical field [0001] The invention relates to the field of spirulina deep processing and biotechnology, in particular to a preparation method of spirulina anti-tumor polypeptide. Background technique [0002] Spirulina (Spirulina platensis) is a kind of cyanobacteria, which has physiological functions such as liver protection, anti-oxidation, anti-virus, anti-cancer and enhancing immunity. One of the highest species. The protein composition of spirulina is closest to the standard stipulated by the World Food and Agriculture Organization (FAO). It contains 18 kinds of amino acids, and has all 8 kinds of amino acids necessary for the human body. It is a high-quality protein source. Available in dry powder and tablet form. Due to its limited solubility, which is not conducive to direct absorption by the human body, its application is limited to a certain extent. Therefore, the separation and purification of active peptides from Spirulina has huge social and economic benefit...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61P35/00
Inventor 张学武许平平张博超
Owner 广东省几何细胞生物科技有限公司
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