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Separation method of yak milk milk fat globule membrane protein

A fat globule membrane protein and separation method technology, which is applied to the preparation methods of peptides, chemical instruments and methods, animal/human peptides, etc. Stabilize, reduce the content of impurity protein, and reduce the effect of loss

Inactive Publication Date: 2013-01-09
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problems of low purity and unstable properties of the milk fat globule membrane protein prepared by the existing method, and provides a method for separating the fat globule membrane protein of yak milk

Method used

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Experimental program
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specific Embodiment approach 1

[0017] Specific embodiment one: the separation method of the yak milk fat globule membrane protein of the present embodiment is carried out according to the following steps:

[0018] 1. Weigh 100 parts of yak milk and 3 to 8 parts of sucrose by mass;

[0019] 2. After filtering the yak milk weighed in step 1 with 3 to 5 layers of gauze, add the sucrose weighed in step 1 and mix evenly to obtain a milk-sucrose mixture;

[0020] 3. Centrifuge the milk-sucrose mixture obtained in step 2 at room temperature to obtain the initial milk fat extract in the upper layer of the centrifuged product; wherein, the centrifugation speed is 3000-4000r / min, and the centrifugation time is 20-35min;

[0021] 4. Add 1 to 3 times the volume of sodium ion phosphate buffer solution to the initial milk fat extract obtained in step 3, and after mixing evenly, perform centrifugal washing at room temperature; The rotating speed is 4200~5200r / min, and the centrifugation time is 15~25min; the preparation ...

specific Embodiment approach 2

[0027] Embodiment 2: This embodiment differs from Embodiment 1 in that: in step 1, 20 parts of yak milk and 1 part of sucrose are weighed in parts by mass. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 3, the centrifugation speed is 3800 r / min, and the centrifugation time is 30 min. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a separation method of yak milk milk fat globule membrane protein, relates to a separation method of protein and solves problems of low purity and unstable properties in terms of cow's milk milk fat globule membrane protein prepared by existing methods. The separation method includes that cane sugar is added in yak milk, a milk fat initial extract is obtained through centrifugation, then centrifugal washing is performed with a sodion phosphate buffer solution and ultrapure water, and finally the centrifugation is performed with a polyethylene glycol octyl phenyl ether solution to obtain the yak milk milk fat globule membrane protein. The prepared yak milk milk fat globule membrane protein has the advantages that the properties are stable, the purity can reach to 93.5%, and problems of low purity and unstable properties in terms of the cow's milk milk fat globule membrane protein prepared by existing separation methods are solved. The separation method is applicable to mass production of the yak milk milk fat globule membrane protein.

Description

technical field [0001] The invention relates to a protein separation method. Background technique [0002] Milk fat is one of the main components of milk. Milk fat is dispersed in milk in the form of fat globules, and its surface is covered by a film with a thickness of 5-10nm, accounting for 2%-6% of the mass of fat globules, called fat globules. Milk Fat Globule Menbrane (MFGM). The milk fat globule membrane is composed of proteins, phospholipids, glycoproteins, triacylglycerols, cholesterol, enzymes and other trace components. Among them, milk fat globule membrane proteins are dominated by glycoproteins, and milk fat globule membrane proteins account for the total milk fat globule membranes. 25%-60% of the mass, accounting for 1%-2% of the total mass of the total milk protein. Studies have shown that milk fat globule membrane protein has good anticancer activity, and breast cancer susceptibility genes extracted from milk fat globule membrane protein have the effect of i...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/14
Inventor 马莺姬晓曦李琳
Owner HARBIN INST OF TECH
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