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Cryptococcus uniguttulatus, and isolated culture method and application thereof

A technology for isolating and culturing Cryptococcus, applied in the biological field

Active Publication Date: 2013-01-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, no Cryptococcus nails have been isolated and purified from lung biopsies and cerebrospinal fluid of patients with meningitis.

Method used

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  • Cryptococcus uniguttulatus, and isolated culture method and application thereof
  • Cryptococcus uniguttulatus, and isolated culture method and application thereof
  • Cryptococcus uniguttulatus, and isolated culture method and application thereof

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Embodiment 1

[0036] The present invention isolates a Cryptococcus uniguttulatus strain CGMCCNo.6017.

[0037] 1. Cryptococcus uniguttulatus strain CGMCC No.6017

[0038] The strain of the present invention (PWH2011) was isolated, detected and identified from the lung tissue and cerebrospinal fluid of cryptococcal meningitis patients by the Dermatology Department of Shanghai Changzheng Hospital. On April 16, 2012, it was deposited in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the preservation registration number is CGMCCNO.6017. Belongs to Basidiomycota, Tremellales, and Filobasidiella.

[0039] 1. Morphological characteristics:

[0040] Ink staining of cerebrospinal fluid showed round yeast-like cells surrounded by thick-walled capsules, such as figure 1 shown.

[0041] 2. Cultivation characteristics:

[0042]The bacteria can grow on malt extract medium (MEA; Oxoid, Basingstoke, UK), canavanine glycine br...

Embodiment 2

[0062] Embodiment 2: fungal drug susceptibility test

[0063] Referring to M27-A3 method (Clinical Laboratory and Standards Institute. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved standard M27-A3. Wayne, PA: National Committee for Clinical Laboratory Standards, 2008.), the Cryptococcus nails The drug sensitivity of amphotericin b, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole and isavuconazole was determined. The MIC values ​​were 0.125, >64, 64, 1, 0.5, 0.5 and 0.5 mg / L, respectively. The results of drug susceptibility showed that the strain was resistant to flucytosine and fluconazole.

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Abstract

The invention relates to the technical field of biology, particularly Cryptococcus uniguttulatus, and an isolated culture method and application thereof. The invention provides a Cryptococcus uniguttulatus strain, which is collected in China General Microbiological Culture Collection Center (CGMCC) on April 16th, 2012, and the collection number is CGMCC No.6017. The invention does further research into morphological characteristics, culture features and carbon source utilization of the Cryptococcus uniguttulatus strain. The invention also provides an isolated culture purification method of the strain, and application of the strain in screening antifungal drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cryptococcus nails and its isolation and cultivation method and application. Background technique [0002] With the continuous improvement of laboratory diagnostic techniques, the classification of Cryptococcus has become more and more systematic, generally divided into: Cryptococcus neoformans, Cryptococcus albicans, Cryptococcus nails, Cryptococcus laurenii, and Cryptococcus xanthophyllum. Cryptococcus nails is an encapsulated basidiomycete yeast that was previously thought to cause onychomycosis only in humans. [Manzano-Gayosso P, Hernández-Hernández F, Méndez-Tovar LJ, et al. Onychomycosis incidence in type 2 diabetes mellitus patients. Mycopathologia. 2008 Jul;166(1):41-5.] [0003] Existing literature also shows that Cryptococcus nails can also cause meningitis or other fungal infections (see literature: Zhang Xuhui, 1 case of Cryptococcus nails meningitis, "Journal of North...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C12Q1/02C12R1/645
Inventor 廖万清潘炜华
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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