Blood cake specimen collecting and treating method and collection card dry blood cake DNA (deoxyribose nucleic acid) extracting method thereof

A technology of specimen collection and processing method, applied in the biological field, can solve the problems of inconvenience in inspection, intolerant of transportation, etc., and achieve the effects of convenient inspection, resistance to transportation, and simple operation

Inactive Publication Date: 2013-01-09
GUANGDONG HYBRIBIO BIOTECH CO LTD
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses sodium citrate or EDTA anticoagulant tubes to preserve venous blood, which needs to be stored in a -20°C refrigerator at low temperature, which is not resistant to transportation and is inconvenient for inspection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood cake specimen collecting and treating method and collection card dry blood cake DNA (deoxyribose nucleic acid) extracting method thereof
  • Blood cake specimen collecting and treating method and collection card dry blood cake DNA (deoxyribose nucleic acid) extracting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] One, carry out blood spot sample collection and processing of the present invention to 22 adults

[0040] 1. Venous blood collection: use sodium citrate to anticoagulate the venous blood, and use a pipette to pipette 80ul of whole blood, and align as figure 1 The center of the blood collection circle of the blood sample collection card shown slowly drips blood onto the collection card to form blood spots and avoid the generation of air bubbles; collect 3 blood circles;

[0041] 2. Collection card blood spot processing

[0042] After the blood sample was collected, the collected card was placed on a dry table for 4 hours to air-dry to form a figure 2 Dried blood spots shown;

[0043] 3. Preservation of Dried Blood Spots on Collection Cards

[0044] After the acquisition cards are air-dried, put each acquisition card into a sealed bag, put a desiccant in the sealed bag, gently squeeze the air out of the sealed bag and seal it, and follow the principle of one bag for o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a blood cake specimen collecting and treating method and a collection card dry blood cake DNA (deoxyribose nucleic acid) extracting method thereof. The blood cake specimen collecting and treating method includes steps of (1), collecting blood; (2), treating collection card blood cakes; (3), preserving the collection card dry blood cakes; and (4), transferring the collection card dry blood cakes. The blood cake specimens can be preserved at normal temperature, easy to transport, convenient to deliver for examination, and can substitute for anti-coagulation blood as DNA extracting specimen for genetic diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for collecting and processing blood spot samples and a method for extracting dried blood spot DNA from a collection card. Background technique [0002] Currently, the DNA used for genetic diagnosis of thalassemia is extracted from anticoagulated whole blood. In this method, sodium citrate or EDTA anticoagulant tubes are used to preserve venous blood, which needs to be stored in a -20°C refrigerator at low temperature, which is not resistant to transportation and is inconvenient for inspection. Therefore, there is an urgent need for a sample collection method that is convenient for transportation and a DNA extraction method with a high extraction rate. Contents of the invention [0003] The object of the present invention is to solve the above problems, and provide a blood spot sample collection and processing method and a collection card dried blood spot DNA extraction me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/10A61B5/15A61B5/153
Inventor 苏彬峰邱美兰卢敏
Owner GUANGDONG HYBRIBIO BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap