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Method for tissue culture of sprout

A tissue culture and seedling technology, applied in the field of agricultural biology, can solve the problems of rare earth elements that have not been reported.

Inactive Publication Date: 2013-01-16
李斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of rare earth elements in promoting the bud formation of Melaleuca alternifolia and preventing browning in subculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Prepare the following four media:

[0017] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium;

[0018] Medium A: Add 7g / L agar, 1.0mg / L6-BA, and 3mg / L neodymium nitrate [Nd(NO 3 ) 3 ];

[0019] Medium B: Add 7g / L agar, 0.01mg / L2, 4-D to the modified MS medium, then add 3mg / L neodymium nitrate [Nd(NO 3 ) 3 ];

[0020] Medium C: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium; then add 1mg / L neodymium nitrate [Nd(NO 3 ) 3 ].

[0021] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four kinds of medium, under the condition of temperature 26°C and light of 2000 lux, light for 12 hours a day, and cultivate for 25 days, the results are as follows:

[0022] When cultured in the control medium CK, the clustered shoots multiplied by 1.8 times, and the subculture browning rate was 25% on the 15th day of culture, and 33% on the 25th day of culture;

[0023] Cultivated in mediu...

Embodiment 2

[0027] 1. Prepare the following four media:

[0028] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium;

[0029] Medium A: Add 7g / L agar, 1.0mg / L6-BA, and 5mg / L lanthanum nitrate [La(NO 3 ) 3 ];

[0030] Medium B: Add 7g / L agar, 0.01mg / L2,4-D to the modified MS medium, then add 5mg / L lanthanum nitrate [La(NO 3 ) 3 ];

[0031] Medium C: Add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium; add 2mg / L lanthanum nitrate [La(NO 3 ) 3 ].

[0032] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four culture media, under the conditions of temperature 25°C and light of 2500 lux, light for 12 hours a day, and cultivate for 27 days, the results are as follows:

[0033] When cultured in the control medium CK, the clustered shoots multiplied by 2.1 times, and the subculture browning rate was 27% on the 15th day of culture, and 34% on the 25th day of culture;

[0034] Cultivated in medium A, th...

Embodiment 3

[0038] 1. Prepare the following four media:

[0039] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium;

[0040] Medium A: Add 7g / L agar, 1.0mg / L6-BA to the modified MS medium, and then add 1mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution;

[0041] Medium B: Add 7g / L agar, 0.01mg / L2,4-D to the modified MS medium, then add 1mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution;

[0042] Medium C: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L2, 4-D to the modified MS medium; then add 0.5mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution.

[0043] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four kinds of medium, under the condition of temperature 25°C and light of 3000 lux, light for 12 hours a day, and cultivate for 20 days, the results are as follows:

[0044] When cultured in the control medium CK, the clustered shoots multiplied by 2...

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Abstract

The invention relates to a method for tissue culture of sprouts, and the method inoculates melaleuca alternifolia adventitious buds on a modified MS medium containing rare earth elements, cytokinin, and auxin for proliferation culture, which can effectively promote the proliferation of clumped buds and prevent the generation of the subculture browning phenomenon. The single rare earth element or mixed rare earth elements comprise nitrates and oxides of lanthanum, cerium, neodymium, praseodymium, and samarium, and the rare earth elements can reduce or substitute the auxin or cytokinin in the medium. The method of the invention has the characteristics of simplicity, easy operation, and economy, can greatly improve the proliferation coefficient of melaleuca alternifolia and effectively prevent the generation of the subculture browning phenomenon.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culturing seedlings. Background technique [0002] In seedling propagation, Melaleuca alternifolia (Me / a / euca / eucadendron) is a long green small tree of the Myrtaceae (Myrtaceae) Melaleuca genus, and the tea tree oil extracted from its fresh branches, leaves and trunks, It is a powerful antibacterial agent and immune activator. It is recognized as an excellent natural health care medical material and is widely used in pharmaceuticals, daily chemicals, food, spices and other industries. Melaleuca alternifolia has the characteristics of fast growth, straight trunk, strong germination, etc. After planting, it can be harvested many times, and has extremely high economic benefits. At present, it has been promoted in many provinces (regions) such as Guangxi, Guangdong, and Fujian. . At present, Melaleuca alternifolia is mainly propagated by se...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李斌
Owner 李斌
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