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Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum

A technology for inducing active, benthic diatoms, applied in the fields of botanical equipment and methods, climate change adaptation, soilless cultivation, etc. Problems such as the development of the sea cucumber industry, to achieve the effect of high possibility, avoid the addition of antibiotics, and increase profits

Inactive Publication Date: 2013-01-16
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of the seed producers are still accustomed to using polyethylene corrugated boards or mesh alone as attachment substrates. The attachment metamorphosis rate of sea cucumber larvae on them is low, generally about 20%, which seriously affects the development of sea cucumber industry.
Benthic diatoms with inductive effects are less used, mainly because it is difficult to determine the species of benthic diatoms with high inductive activity
There has not yet been a substrate for sea cucumber larvae with a high rate of metamorphosis optimized for benthic diatoms with high induction activity.

Method used

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  • Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum
  • Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum

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Embodiment 1

[0020] Example 1. Preparation of the attachment base of the present invention.

[0021] The main implementation steps of this patent are divided into: (1) screening of highly induced benthic diatom strains; (2) large-scale cultivation of benthic diatoms; (3) pretreatment of attachment media; (4) utilization of high induction Active benthic diatom membranes optimize attachment substrates.

[0022] (1) Screening technology for benthic diatom strains with high induction activity.

[0023] Collect microbial films from the surface of sea cucumber culture ponds and rocks in seawater, centrifuge to obtain sediment, add sterilized seawater several times and centrifuge, use conventional benthic diatom solid medium (f / 2 medium, plus 1.5% Agar) for 48 hours of plate culture, the algal strains that formed monoclonals were plated twice through microscope observation, and finally the unicellular benthic diatom strains were isolated.

[0024] The isolated unicellular diatoms were inoculate...

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Abstract

The invention belongs to the field of marine aquaculture, and particularly relates to a sea cucumber larvae attachment substratum adhered with a benthic diatom membrane with high induction activity and a preparation method of the sea cucumber larvae attachment substratum. The sea cucumber larvae attachment substratum comprises the attachment substratum, wherein the benthic diatom membrane with high induction activity is arranged on the substratum. The preparation of the attachment substratum comprises steps of screening benthic diatom strains with high induction activity; culturing the benthic diatom on a large scale; pre-treating the attachment substratum; and optimizing the attachment by the benthic diatom with high induction activity. The sea cucumber larvae attachment substratum provided by the invention is capable of effectively increasing the attachment rate of sea cucumber larvae by higher than 50%, and the benthic diatom used as a food source for the sea cucumber larvae is capable of guaranteeing later growth of the sea cucumber larvae; and the increase of the attachment of the sea cucumber larvae is capable of avoiding the addition of antibiotics in a seedling production process so as to achieve a clean production effect.

Description

technical field [0001] The invention belongs to the field of mariculture, and in particular relates to a sea cucumber seedling attachment base attached with a benthic diatom membrane with high inductive activity and a preparation method thereof. Background technique [0002] The metamorphosis process of sea cucumber larvae from planktonic salivary larvae to attached five-tentacle larvae is the most important link in the development of sea cucumbers, and it is also the key to the success of sea cucumber seedlings. The attachment substrate is an indispensable and important condition for sea cucumber larvae to achieve attachment metamorphosis. The currently commonly used attachment bases are mainly polyethylene corrugated boards, polyethylene mesh sheets, etc., and there are many patents on the attachment bases for sea cucumber larvae, such as the attachment base for sea cucumber larvae with the patent number ZL200720013117.9. At present, the low rate of metamorphosis of larva...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01G31/00
CPCY02A40/81Y02P60/60
Inventor 包卫洋杨美圆孙景春张力封克
Owner YANGZHOU UNIV
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