Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum

A technology for inducing active, benthic diatoms, applied in the fields of botanical equipment and methods, climate change adaptation, soilless cultivation, etc. Problems such as the development of the sea cucumber industry, to achieve the effect of high possibility, avoid the addition of antibiotics, and increase profits

Inactive Publication Date: 2013-01-16
YANGZHOU UNIV
6 Cites 1 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0003] However, most of the seed producers are still accustomed to using polyethylene corrugated boards or mesh alone as attachment substrates. The attachment metamorphosis rate of sea cucumber larvae on them is low, generally about 20%, which seriously affects the development of sea cucumber industry.
Ben...
View more

Method used

Conventional attachment bases such as polyethylene corrugated plate or mesh are soaked in the sodium hypochlorite aqueous solution of not less than 50 mg/l and soaked 1-2 days, adopt running water to rinse 1-2 time, reuse the s...
View more

Abstract

The invention belongs to the field of marine aquaculture, and particularly relates to a sea cucumber larvae attachment substratum adhered with a benthic diatom membrane with high induction activity and a preparation method of the sea cucumber larvae attachment substratum. The sea cucumber larvae attachment substratum comprises the attachment substratum, wherein the benthic diatom membrane with high induction activity is arranged on the substratum. The preparation of the attachment substratum comprises steps of screening benthic diatom strains with high induction activity; culturing the benthic diatom on a large scale; pre-treating the attachment substratum; and optimizing the attachment by the benthic diatom with high induction activity. The sea cucumber larvae attachment substratum provided by the invention is capable of effectively increasing the attachment rate of sea cucumber larvae by higher than 50%, and the benthic diatom used as a food source for the sea cucumber larvae is capable of guaranteeing later growth of the sea cucumber larvae; and the increase of the attachment of the sea cucumber larvae is capable of avoiding the addition of antibiotics in a seedling production process so as to achieve a clean production effect.

Application Domain

Climate change adaptationAgricultural fishing +3

Technology Topic

SeawaterSea cucumber +6

Image

  • Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum
  • Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum

Examples

  • Experimental program(1)

Example Embodiment

[0020] Example 1. Preparation of the attachment base of the present invention.
[0021] The main implementation steps of this patent are divided into: (1) screening of highly inducible benthic diatom strains; (2) large-scale cultivation of benthic diatoms; (3) pretreatment of attachment base; (4) utilization of highly induced diatoms The active benthic diatom membrane optimizes the attachment base.
[0022] (1) Screening technology for benthic diatom strains with high inducing activity.
[0023] Collect microbial membranes from the surface of sea cucumber farming ponds and rocks in the seawater, centrifuge to obtain the sediment, add sterile seawater for many times, and centrifuge, use conventional benthic diatom solid medium (f/2 medium, plus 1.5% Agar) was cultured for 48 hours, and the single-celled algae strains were subjected to a second streak through microscope observation, and the single-cell benthic diatom strains were finally isolated.
[0024] The separated single-celled diatoms were inoculated into a conventional f/2 liquid medium and cultured for 7 days. The attached benthic diatoms were scraped off, centrifuged and concentrated, and the benthic diatoms were allowed to form a diatom film in a petri dish. Add mature sea cucumber larvae to the petri dish, and observe the attachment rate of the larvae under a microscope after 48-72 hours. Utilize the attachment rate of larvae (> 80%) Benthic diatom strains with high inducing activity were detected.
[0025] Indoor experiments screened more than 20 species of benthic diatoms with good attachment performance, and tested the effect of larval attachment metamorphosis induction (with figure 1 9 kinds in the display as examples). As the density of diatoms increases, the inducing activity of benthic diatom biofilms (an indicator of the rate of attachment and metamorphosis of larvae) presents different patterns. Among them, the inducing activity of S1 (identified as Nitzschia) is as high as 85%. Without diatom biofilm (indicated by the black line), the attachment metamorphosis rate of larvae is only 11%.
[0026] (2) Large-scale cultivation technology of benthic diatoms.
[0027] A large 2-liter Erlenmeyer flask was used to conduct preliminary large-scale cultivation of the highly active benthic diatoms, and the algae liquid was transferred to the nursery pond after 72 hours. Add the conventional f/2 diatom culture medium to the nursery pond to continuously cultivate the diatoms on a large scale under aeration. After 5-7 days, they will be in the exponential growth phase, and the density will reach the million level.
[0028] (3) Pretreatment technology of attachment base.
[0029] Soak conventional attachment bases such as polyethylene corrugated board or mesh in a sodium hypochlorite aqueous solution of not less than 50 mg/l for 1-2 days, rinse with tap water 1-2 times, and then rinse with seawater for seedlings 3 times to remove Contaminants on the surface of polyethylene corrugated board or mesh, as well as residual sodium hypochlorite, ensure the cleanliness of the attachment base.
[0030] (4) Optimize the attachment base technology by using highly induced active benthic diatoms.
[0031] Put the treated clean attachment base into the diatom culture tank, stop the aeration or slightly aerate, after 48-72 hours, the benthic diatom will attach and grow on the surface of the attached base to form a benthic diatom film. Drain the f/2 diatom culture medium in the nursery pond, rinse the nursery pond with nursery water, and then fill the nursery pond with seawater.
[0032] Nitzschia ( figure 1 S1) The corrugated board is optimized, that is, the benthic diatom biofilm is cultivated on the corrugated board. The application effect in the nursery shows that the benthic diatom biofilm attachment base significantly increases the attachment density of the larvae. Such as figure 2 , The attachment density of larvae on the substrate of benthic diatom biofilm is 90 per dm 2 , And the adhesion density on the corrugated board without optimization is only 58 pcs/dm 2 , The attachment efficiency of larvae increased by 55% (in actual operation, because the cultivation time of benthic diatoms is shorter, the density of diatoms in the diatom biofilm is lower. If the density of diatoms is further increased, the attachment efficiency of larvae will be higher).

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Similar technology patents

Loss control nitrogenous fertilizer and producing method thereof

Owner:INST OF PLASMA PHYSICS CHINESE ACAD OF SCI

Cloud computing resource management system and method

InactiveCN102185926ALower rental bidsincrease profit
Owner:SHENGDA COMP SHANGHAI

A communication method and load balancer

ActiveCN102265568ALower Bandwidth Requirementsincrease profit
Owner:HUAWEI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products