Unlock instant, AI-driven research and patent intelligence for your innovation.

Immobilization method of heparinase III

A technology of heparinase and chitosan microspheres, which is applied in the same direction of being immobilized on/in an organic carrier

Active Publication Date: 2014-09-03
SHENZHEN HEPALINK PHARMA GRP CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the immobilization of heparanase Ⅲ

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] The heparinase used is isolated and purified from the fermented and cultured cells of Flavobacterium heparinus. For the enzyme preparation process, refer to the example in "A Preparation Method for Flavobacterium Heparinus Heparinase III" of Invention Patent Application No. 200910039360.1.

[0040] a, getting 1g of commercially available chitosan powder with a purity of 99% is dissolved in 50ml of 2% acetic acid to obtain an acidic chitosan sol, and 0.6g of commercially available chemically pure Span 80 (Sinopharm Chemical Reagent Co., Ltd. , chemically pure) was added into 100ml of liquid paraffin, stirred and mixed at a speed of 800rpm, the acidic chitosan sol was added dropwise into the liquid paraffin, after emulsification for 1h, 5ml of glutaraldehyde with a mass fraction of 25% was added as a crosslinking agent, The crosslinking temperature is 40°C, add 100ml equal volume of 2.5M NaOH and absolute ethanol mixture after 1h, let it stand for 10min after stirring, dis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention relates to an immobilization method of heparinase III, and particularly relates to a method for immobilizing heparinase III by taking chitosan microspheres as an immobilization material. The immobilized heparinase III has the advantages that the immobilized heparinase III is recyclable and reusable and enzymes can be easily separated from substrates and products and the like.

Description

technical field [0001] The invention relates to a method for immobilizing heparinase III, in particular to a method for immobilizing heparinase III by using chitosan as an immobilizing material, so as to realize the immobilization and reusability of heparinase III . Background technique [0002] Heparanase refers to a class of enzymes that can specifically cleave the glycosidic bonds of heparin and heparin-like main chains. It was first discovered and isolated from Flavobacterium heparinus, and later found that heparinases also exist in some microorganisms and animal tissues. exist. At present, there are about 10 kinds of heparinases reported in academic papers, and only three enzymes from Flavobacterium heparinus have been studied in detail, namely heparinase Ⅰ (EC number 4.2.2.7), heparanase Ⅱ (no EC number), heparanase III (EC number 4.2.2.8). Heparanase Ⅰ, Ⅱ, Ⅲ are monomeric proteins with molecular weights of about 43, 78, and 66 kd, respectively, and their isoelectri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10
Inventor 马小来白佳珂史绍鹏李锂
Owner SHENZHEN HEPALINK PHARMA GRP CO LTD