Preparation method of hydrogel for three-dimensional culture of tumor cells

Abstract

The invention discloses a preparation method of a hydrogel for the three-dimensional culture of tumor cells. A methyl vinyl ether / maleic acid copolymer which is a polymethyl vinyl ether / maleic anhydride hydrolysate is cross-linked by a cross-linking agent which is polyethylene glycol, a poly(lactide-co-glycolide)-polyethylene glycol-poly(lactide-co-glycolide) triblock copolymer, a poly-epsilon-caprolactone lactide-polyethylene glycol-poly-epsilon-caprolactone lactide triblock copolymer or glucan; the proportion of a carboxyl group in the methyl vinyl ether / maleic acid copolymer and the proportion of a hydroxy group in the cross-linking agent are adjusted to obtain a gel of different cross-linking densities, superfluous carboxyl groups in the gel after the cross-linking are esterified by polyethylene glycol monomethyl ether; the obtained gel is processed to prepare a 5% aqueous solution; and the aqueous solution is neutralized with a weak alkali such as a saturated aqueous solution of sodium bicarbonate to the pH value of 7.0 in order to prepare the hydrogel which can be used as a tumor cell three-dimensional culture medium.

Description

technical field

[0001] The invention relates to the field of preparation of medical materials, in particular to a method for preparing a hydrogel applicable to three-dimensional culture of tumor cells and high-throughput screening of antitumor drugs.

[0002] Background technique

[0003] Traditional two-dimensional tumor cell culture (2D culture) is widely used in tumor cell culture due to its simplicity, ease of operation, and low cost. However, due to the large difference between 2D culture and the real environment in vivo, such as tumor cells growing in a plane, a three-dimensional environment cannot be constructed, and the interaction between cells and cells or between cells and the matrix cannot be truly reflected. The in vitro three-dimensional cell culture (3D culture) microenvironment is closer to the cell growth environment in vivo, so 3D culture has been widely used in the biomedical field since the beginning of the 21st century. Applying 3D culture technolo...