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Duck hemorrhagic ovaritis virus

A technique for hemorrhagic oophoritis, virus, applied in the field of animal virology

Active Publication Date: 2013-01-30
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Because it is a new infectious disease, there is still a big gap in the research on the isolation, identification, biological characteristics and main antigen gene characteristics of duck hemorrhagic ovariitis virus (DHOV)

Method used

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  • Duck hemorrhagic ovaritis virus
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Experimental program
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Embodiment Construction

[0020] 1. Virus Isolation

[0021] (1) Treatment of diseased materials The inventors collected from diseased ducks in a laying duck farm. Cut 5.0g of tissue by aseptic operation, add a small amount of MEM (including penicillin and streptomycin 1000U / mL), grind until it becomes a paste, then add 4 times the volume of MEM to dilute into a suspension, and store the sample at -80°C and room temperature Freezing and thawing was repeated 3 times, then centrifuged at 4°C and 12000r / min for 10min, the collected supernatant was filtered and sterilized with a 0.22μm disposable filter, and finally aliquoted and stored at -80°C for later use.

[0022] (2) Chicken embryo fibroblasts (CEF) were isolated from 9-day-old SPF chicken embryos (both SPF chicken embryos and SPF laying hens were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.). The viscera were cut into pieces with ophthalmic scissors, and then digested with 0.25% trypsin. After the liquid was natural...

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Abstract

The invention relates to a duck hemorrhagic ovaritis virus (DHOV). Tissue samples of clinical diseased egg ducks are finely separated by SPF (specific pathogen free) chicken embryo fibroblasts (CEF) to obtain a DHOV-JN, and the isolate can proliferate on CEF and produce a typical cytopathoenic effect (CPE), and can also proliferate one MDCKMadin-Darby canine kidney, Vero, BHK-21(baby hamster kidney-21), ST (Swine Testis) and Marc-145 cell monolayer and produce more consistent CPE, and the tilter of the isolate is 105.5TCID5(Tissue Culture Infectious Dose)50 / 0.1mL; the isolate belongs to a member of flaviviridae, yellow disease and Ntaya virus group; and the strain can develop corresponding RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection / diagnosis methods (reagents) based on the study, and can also develop vaccines and other biological products for clinical prevention of the disease.

Description

technical field [0001] The invention relates to a strain of duck hemorrhagic oophoritis virus. The virus was isolated from a new duck disease. It belongs to the field of animal virology. Background technique [0002] Since the beginning of April 2010, a kind of epidemic disease characterized by a rapid decline in egg production rate, even production stoppage and death in varying degrees has occurred in breeding ducks and laying ducks in Zhejiang, Jiangsu, Shandong, Fujian, Hebei, Beijing and other provinces in China, commonly known as breeding ducks. (Eggs) duck egg production drop, ovarian hemorrhage, the virus that caused this disease was originally named duck flavivirus virus (DFV), and now it is basically named duck hemorrhagic ovariitis virus (Duck hemorrhagic hvaritis virus, DHOV) ). All varieties of laying ducks and breeding ducks have occurred, but it is mainly seen in laying ducks. The egg production rate of laying ducks was low for a long time (40-60 days) afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
Inventor 邹敏吴发兴刘东刘新文申洪银孙健刘蕾范根成杜元钊
Owner YEBIO BIOENG OF QINGDAO