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Modified polystyrene material with high cell adhesion capacity and preparation method of modified polystyrene material

A technology of polystyrene and cell adhesion, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of expensive, unfavorable large-scale production, etc., and achieve improved efficiency and simple operation , No secondary pollution source effect

Inactive Publication Date: 2013-02-06
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, grafted substances such as peptides are often expensive, which is not conducive to large-scale production

Method used

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  • Modified polystyrene material with high cell adhesion capacity and preparation method of modified polystyrene material
  • Modified polystyrene material with high cell adhesion capacity and preparation method of modified polystyrene material
  • Modified polystyrene material with high cell adhesion capacity and preparation method of modified polystyrene material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, preparation modified polystyrene culture plate

[0024] Put a commercially available common polystyrene cell culture plate in an air atmosphere, put it into a commercially available medical sealed bag, and seal it with the self-applied glue of the sterilization tape, and then use it 60 Coγ-rays were irradiated in air at room temperature, the radiation dose rate was 30Gy / min, and the absorbed dose was 50kGy; after the irradiation was completed, a modified polystyrene culture plate was obtained.

[0025] The photos of modified polystyrene prepared in this embodiment and unmodified polystyrene are as follows: figure 1 shown. Depend on figure 1 It can be seen that the appearance of modified polystyrene has no obvious change from that before modification.

[0026] The water contact angles of modified polystyrene prepared in this embodiment and unmodified polystyrene are as follows: figure 2 shown. Depend on figure 2 It can be seen that the surface conta...

Embodiment 2

[0030] Embodiment 2, preparation modified polystyrene culture plate

[0031] The preparation process of this example is basically the same as that of Example 1, except that the radiation dose rate is changed to 50Gy / min, and the absorbed dose is changed to 100kGy.

[0032] Observe the modified polystyrene material prepared in this example according to the method for measuring the appearance of the material in Example 1. Such as figure 1 As shown, the overall color of the modified polystyrene prepared in this embodiment is more yellow. It shows that the color of modified polystyrene gradually turns yellow with the increase of irradiation dose.

[0033] Observe the modified polystyrene material prepared in this example according to the method for measuring the surface contact angle of water in the material in Example 1. Such as figure 2 As shown, the surface contact angle of the modified polystyrene prepared in this example to water is lower to some extent than that before ...

Embodiment 3

[0036] Embodiment 3, preparation modified polystyrene culture plate

[0037] The preparation process of this example is basically the same as that of Example 1, except that the radiation dose rate is changed to 100Gy / min, and the absorbed dose is changed to 500kGy.

[0038] Observe the modified polystyrene material prepared in this example according to the method for measuring the appearance of the material in Example 1. Such as figure 1 As shown, the overall color of the modified polystyrene prepared in this embodiment is more yellow. It shows that the color of modified polystyrene turns yellow and darkens with the increase of irradiation dose.

[0039] Observe the modified polystyrene material prepared in this example according to the method for measuring the surface contact angle of water in the material in Example 1. Such as figure 2 As shown, the surface contact angle of the modified polystyrene prepared in this example to water is lower to some extent than that befo...

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Abstract

The invention discloses a modified polystyrene material with a high cell adhesion capacity and a preparation method of the modified polystyrene material. The preparation method of the modified polystyrene material includes the following materials of subjecting a polystyrene material to sealed packaging by using a sterilization bag, and irradiating the material by using gamma-rays or electron beams to obtain the modified polystyrene material with the high cell adhesion capacity. By means of the high-dose gamma-ray modified polystyrene material, cell adhesion efficiencies can be remarkably improved, production of polystyrene cell culture boards with higher performances is facilitated; and the high-dose gamma-rays are used for processing the polystyrene culture boards, cell adhesion is enhanced by the modified material, simultaneously, the material is sterilized and disinfected, and the modified material can be directly applicable to cell culture. The modified method is simple in operation, efficient, economical, free of a secondary pollution source and suitable for large-scale application in the industry.

Description

technical field [0001] The invention relates to a modified polystyrene material with high cell adhesion ability and a preparation method thereof. Background technique [0002] Polystyrene has been widely used as a cell culture plate because of its good light transmission performance, good strength and easy plasticity, and non-toxicity. However, the surface of polystyrene is hydrophobic, which is not conducive to cell adhesion. Since most cells need to adhere to a solid interface to grow. The use of culture plates with stronger cell adhesion can reduce cell culture time, shorten the experimental cycle, and efficiently proliferate specific cells, which is of great significance in cell biology. At present, there are many methods to promote cell adhesion on polystyrene surface, among which surface coating and surface grafting are the most widely used methods. For example: US patent application US13255299 adsorbs or grafts polypeptides on the surface of polystyrene to promote ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/00C08L25/04C12M3/00
Inventor 魏世成赵荧晖刘燕周彦恒
Owner PEKING UNIV
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