Triterpenoid saponin anti-myocardial ischemia compound

A triterpenoid saponin, anti-myocardial ischemia technology, applied in the field of medicine, can solve problems such as chemical structure and anti-myocardial ischemia activity that have not been reported, and achieve the effect of alleviating adverse reactions and drug resistance

Active Publication Date: 2013-02-13
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We systematically separated the total saponins of Clematis clematis, and obtained a new triterpenoid saponin compound, whose chemical structure and anti-myocardial ischemic activity have not been reported before

Method used

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  • Triterpenoid saponin anti-myocardial ischemia compound
  • Triterpenoid saponin anti-myocardial ischemia compound
  • Triterpenoid saponin anti-myocardial ischemia compound

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: Extraction and separation of compounds

[0017] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into a coarse powder, take 3 kg, add 15 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 3 kg. Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 540 g of ethanol extract. The extract was dispersed in 4.5 liters of water, and extracted 3 times with petroleum ether, 4.5 liters each time. The extracted aqueous phase was then extracted 3 times with n-butanol, 4.5 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 120 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the elue...

Embodiment 2

[0022] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into coarse powder, take 3 kg, add 12 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 2 Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 510 g of ethanol extract. The extract was dispersed in 3 liters of water, and extracted 3 times with petroleum ether, 3 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 3 liters each time, the n-butanol extract was combined, and the solvent was recovered under reduced pressure to obtain 115 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water mixed solvent, and 1...

Embodiment 3

[0024]The whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province was collected as a raw material. After the raw material was crushed into a coarse powder, 1 kg was taken, and 3 liters of methanol were added for reflux extraction. A total of 3 extractions were performed for 1 hour each time. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 150 g of methanol extract. The extract was dispersed in 0.6 liters of water, and extracted 3 times with petroleum ether, 0.6 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 0.6 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 45 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water ...

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Abstract

The invention provides a triterpenoid saponin anti-myocardial ischemia compound. The molecular formula is C66H108O32; and by adopting a chemical method and spectrum technologies such as high resolution mass spectrum and dimensional nuclear magnetic resonance spectrum, the chemical structure of the compound is determined as hederagenin 28-O-alpha-L-rhamnopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranoside. In-vitro anti-myocardial ischemia activity results show that the compound has a treatment effect on hypoxia / reoxygenation model-induced myocardial cell damage. The compound can become a new medicament for treating cardiovascular diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a triterpene saponin anti-myocardial ischemia compound, specifically a new triterpene saponin compound extracted and separated from the Tibetan medicine Clematis clematis and its preparation method Applications in cardiovascular medicine. Background technique [0002] Ischemic heart disease is the main cause of death in humans, and its high morbidity and mortality seriously affect the quality of life of patients and are life-threatening. At present, the drugs for clinical treatment of cardiovascular diseases mainly include nitrates, calcium antagonists and β receptor blockers. Although these drugs have achieved certain clinical curative effects, they all have different degrees of toxic and side effects and drug resistance. Therefore, the development of new efficient and safe cardiovascular drugs is an urgent need for the treatment of this disease. [0003] Clematis tangutica, a plant o...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P9/10
Inventor 文爱东汤海峰张伟魏海东奚苗苗姚敏娜段佳林
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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