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Enzyme hydrolysis method for preparing fetus cervi active peptide

A technology of active polypeptide and enzymatic hydrolysis, which is applied in the fields of food science and bioengineering, can solve the problems of rough processing of deer fetus products, waste of deer fetus resources, and less research on deer fetus, so as to enhance the body's immunity, save industrial costs, Good inhibitory effect

Active Publication Date: 2013-02-13
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic and foreign studies on sheep fetuses, cow fetuses, pig fetuses, etc. are relatively in-depth, while studies on deer fetuses are less.
The application of deer fetus in my country is very primitive. The processing of deer fetus products is rough, the products are primary, the types are single, and the curative effect is not significant, resulting in a great waste of deer fetus resources
Although there are also methods for studying the deep processing of deer fetuses that have been disclosed, they use the acetone extraction method, and there is no report on the method of extracting active polypeptides from deer fetuses by enzymatic hydrolysis using modern bio-enzyme engineering technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Preparation of Deer Fetus Active Polypeptide by Enzymolysis

[0013] 1) Remove tendons and membranes from fresh deer placenta, cut into pieces; grind in a grinder, fully homogenate; freeze at -40~-20°C, thaw at room temperature, freeze and thaw repeatedly 3 to 5 times, centrifuge, and the supernatant is Deer placenta extract, sediment for later use;

[0014] 2) Take the deer placenta extract, preheat at 30°C for 10 minutes, adjust the pH value to 1.5, add pepsin at 35°C at an amount of 1600u / ml, enzymatically react for 5 hours, and maintain the enzymatic hydrolyzate during the reaction The pH is 1.5 and the temperature is 35°C. After the reaction, heat sterilization at 65°C for 30 minutes, centrifuge at 8000 r / min for 15 minutes, and take the supernatant, which is the deer fetus active polypeptide extract;

[0015] 3) The obtained deer placenta active polypeptide extract was freeze-dried to obtain deer placenta active polypeptide dry powder, and the yield wa...

Embodiment 2

[0016] Example 2 Preparation of Deer Fetus Active Polypeptide by Enzymolysis

[0017] 1) Remove tendons and membranes from fresh deer placenta, cut into pieces; grind in a grinder, fully homogenate; freeze at -40~-20°C, thaw at room temperature, freeze and thaw repeatedly 3 to 5 times, centrifuge, and the supernatant is Deer placenta extract, sediment for later use;

[0018] 2) Take the deer placenta extract, preheat at 35°C for 10 minutes, adjust the pH value to 7.5, add bromelain at 50°C at an amount of 1600u / ml, enzymatically react for 8 hours, and maintain the enzymatic hydrolyzate during the reaction The pH is 7.5 and the temperature is 50°C. After the reaction, it is heated and sterilized at 65°C for 30 minutes, centrifuged at 8000 r / min for 15 minutes, and the supernatant is taken, which is the deer fetus active polypeptide extract;

[0019] 3) The obtained deer fetus active polypeptide extract was freeze-dried to obtain deer fetus active polypeptide dry powder, and ...

Embodiment 3

[0021] Example 3 Preparation of Deer Fetus Active Polypeptide by Enzymolysis

[0022] 1) Remove tendons and membranes from fresh deer placenta, cut into pieces; grind in a grinder, fully homogenate; freeze at -40~-20°C, thaw at room temperature, freeze and thaw repeatedly 3 to 5 times, centrifuge, and the supernatant is Deer placenta extract, sediment for later use;

[0023] 2) Take the deer placenta extract, preheat it at 35°C for 10 minutes, adjust the pH value to 8.0, add papain at 45°C in an amount of 1600u / ml, and perform enzymatic hydrolysis for 8 hours, and maintain the enzymatic hydrolysis solution during the reaction The pH is 8.0 and the temperature is 45°C. After the reaction, it is heated and sterilized at 65°C for 30 minutes, centrifuged at 8000 r / min for 15 minutes, and the supernatant is taken, which is the deer fetus active polypeptide extract;

[0024] 3) The obtained deer placenta active polypeptide extract was freeze-dried to obtain deer placenta active p...

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PUM

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Abstract

The invention relates to an enzyme hydrolysis method for preparing fetus cervi active peptide. The technical scheme adopted by the invention is that the enzyme hydrolysis method comprises steps as follows: getting a fresh fetus cervi placenta; removing rib and membrane; crushing by shearing; grinding by a grinding machine; fully homogenizing; freezing at a temperature of -40 to -20 DEG C; melting at a room temperature; repeatedly freezing and melting for three to five times; centrifuging; getting supernate as fetus cervi placenta extracting liquid; getting precipitate for standby; preheating the fetus cervi placenta extracting liquid for 10 to 15 minutes at the temperature of 30 to 35 DEG C; adjusting the pH (Potential Of Hydrogen) to reach 1.5 to 7.5; adding enzyme to carry out enzyme hydrolysis for 5 to 8 hours at the temperature of 35 to 50 DEG C; heating at the temperature of 65 DEG C to sterilize for 30 minutes; centrifuging; then getting the supernate which is the fetus cervi active peptide extracting liquid; freezing and drying the fetus cervi active peptide to obtain dried fetus cervi active peptide powder. By adopting the enzyme hydrolysis method for preparing the fetus cervi active peptide, more active peptide substances can be extracted from fetus cervi, so that waste polysaccharide and lipid resource can be fully utilized, processing cost can be reduced, and environmental pollution can be avoided at the same time.

Description

technical field [0001] The invention belongs to the fields of food science and bioengineering, and in particular relates to a method for preparing deer placenta active polypeptide by using biological protease to obtain a new product with biological activity. Background technique [0002] Deer fetus refers to the fetus with afterbirth and fetal fluid in the abdomen of the doe, or the fetus with no hair on the body surface, fasting, and complete development of various organs in the body, but the fetus is aborted due to mechanical reasons or due to lack of milk or refusal by the doe after delivery. Young deer that died due to feeding, addiction, etc. The pharmacological effects of deer fetus were first published in "Compendium of Materia Medica", saying that it can benefit kidney yang and nourish essence and blood. Placenta has the effect of enhancing resistance and immunity in immunology. Modern biological and medical research has proved that the placenta contains a variety ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 胡风庆倪裴回晶
Owner LIAONING UNIVERSITY
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