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Method for decreasing beta-lactoglobulin in concentrated whey albumen powder through biotic compound protein enzymolysis

A technology of compound protease and whey concentrate, which is applied in the field of biological compound protease hydrolysis to reduce β-lactoglobulin in whey protein concentrate powder, can solve the problems of increase of β-lactoglobulin and different composition, and achieve the effect of reducing allergies

Active Publication Date: 2013-02-27
贝因美(杭州)食品研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the whey protein composition of breast milk and bovine milk is different. The whey protein of breast milk is mainly α-lactalbumin, almost without β-lactoglobulin; while the main protein in bovine whey is β-lactoglobulin (43~50 %), the addition of desalinated bovine whey powder resulted in an increase in β-lactoglobulin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The method for reducing β-lactoglobulin in concentrated whey protein powder by biological complex proteolysis, the method includes the following steps:

[0020] (1) Dissolve, heat and keep warm: Dissolve a certain amount of concentrated whey protein powder in water with a mass ratio of 1.6~5.0%, and keep the solution at 50~100℃ for 20~60min, and then cool to 45℃;

[0021] (2) Enzymatic hydrolysis: Add complex enzymes to the whey protein solution, and the mass ratio of concentrated whey protein powder to complex enzymes is (40:~80): 1,30~45℃ for 3~8 hours; Compound enzymes include trypsin, neutral protease and papain. The mass ratio of trypsin: neutral protease: papain is (8~10):1:(0.5~1.0), trypsin activity ≥2500U / mg, neutral Protease activity ≥50U / mg, papain activity ≥3U / mg. Trypsin, neutral protease and papain are compounded in proportion to have strong hydrolysis ability for β-lactoglobulin in concentrated whey protein powder.

[0022] (3) Evaporation and conce...

Embodiment 2

[0024] Example 2: The difference from Example 1 is that the solution is kept at 100°C for 20-60 minutes and then cooled to 45°C; the mass ratio of concentrated whey protein powder to complex enzyme is 40:1, trypsin: neutral The mass ratio of protease: papain is 8:1:0.5.

Embodiment 3

[0025] Example 3: The difference from Example 1 is that the mass ratio of concentrated whey protein powder to the complex enzyme is 80:1, and the mass ratio of trypsin:neutral protease:papain is 10:1:1.

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Abstract

The invention provides a method for decreasing beta-lactoglobulin in concentrated whey albumen powder through biotic compound protein enzymolysis. The method comprises the following steps of: (1) dissolving, heating and preserving heat: a certain amount of concentrated whey albumen powder is dissolved in water, the mass ratio of concentrated whey albumen powder is 1.6-5.0 percent, the solution is subjected to heat preservation at the temperature of 50-100 DEG C for 20-60min, and the solution is cooled to 45 DEG C; (2) carrying out enzymolysis: compound enzyme is added into whey albumen liquid, the mass ratio of the concentrated whey albumen powder to the compound enzyme is (40-80):1, and enzymolysis is carried out at the temperature of 30-45 DEG C for 3-8 hours; (3) evaporating and concentrating: a sterilization treatment is processed before concentration, the temperature of sterilization is controlled to be 100-125 DEG C, the time for heat preservation is 90-180s, the temperature of concentration is controlled to be 50-85 DEG C, and the concentration of solid materials after concentration is 25-35 percent; and (4) atomizing and drying: the air inlet temperature of hot wind is 200-210 DEG C, the air outlet temperature of hot wind is 80-90 DEG C, and the material liquid temperature is 65-75 DEG C. The method has the advantages that 1, high quality ingredient of lactoglobulin and the like in cow milk is persisted, and the category and the proportion of amino acid are also persisted; and 2, a part of peptide is generated through hydrolysis (enzymolysis), thus the phenomenon that an infant is allergic to lactoprotein is more facilitated to be reduced.

Description

Technical field [0001] The invention relates to the field of whey protein powder, and is mainly a method for reducing β-lactoglobulin in concentrated whey protein powder by biological composite proteolysis. Background technique [0002] The health of infants and young children is related to the future of a nation and a country. Scientific research shows that the nutritional status of infants and young children lays the foundation for physique and intelligence in adulthood. Breast milk is the most ideal natural food for babies. Breast milk contains various nutrients necessary for the growth and development of infants and young children. It is extremely important for the survival, growth, development, health and nutrition of infants and young children, and has the highest bioavailability. In addition, breast milk is also rich in immunologically active substances, which can significantly improve the baby's resistance and prevent allergies. At present, there are three main ways of f...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/08
Inventor 何光华黄俊储小军魏培莲华家才尤玉如
Owner 贝因美(杭州)食品研究院有限公司
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