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Preparation method of bacillus amyloliquefaciens S4 for fermentation of tobacco straw bio-organic fertilizer

A bio-organic fertilizer and fermentation strain technology, which is applied in the field of strain preparation of tobacco straw bio-organic fertilizer by solid-state fermentation, can solve the problems of no optimal selection of microbial strains, ineffective fermentation transformation, and poor effect, etc., to achieve increased Yield and, improve internal quality, increase the effect of output value

Inactive Publication Date: 2013-02-27
中国烟草总公司湖北省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, there is a method of fermenting tobacco stalks into bio-organic fertilizers by borrowing the solid fermentation technology of common crop stalks. However, because tobacco stalks contain a large amount of nicotine and contain a large number of harmful bacteria and viruses, this method does not preferably select microorganisms. species, the effect is not good
Commonly used fermentative bacteria are not suitable for survival under the condition of a large amount of nicotine substances, and cannot carry out effective fermentation transformation, so the existing methods are difficult to solve the above problems

Method used

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  • Preparation method of bacillus amyloliquefaciens S4 for fermentation of tobacco straw bio-organic fertilizer

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Embodiment 1

[0029] Strain culture: take the above S 4 1 bacterium tube, insert in the 250ml seed bottle that fills culture medium, and its composition of described culture medium is: glucose 1%, peptone 0.8%, yeast extract 0.5%, sodium chloride 0.4%, all the other are water. Adjust the pH to 7.8.

[0030] Incubate a 250ml seed bottle for 20 hours at 28±1°C with shaking, and S 4 The bacterial solution is added to a 1000ml fermentation bottle according to the seed amount of 6% volume ratio; under the condition of 28±1°C and shaking, the 1000ml fermentation bottle is cultivated for 24 hours, and the OD600nm value is measured to obtain the fermentation bacterial agent.

Embodiment 2

[0032] Strain culture: take the above S 4 1 bacterial tube and D of divisional application 1 3 bacterial tubes, respectively inserted into 250ml seed bottles filled with culture medium, the composition of the culture medium is: 0.8% glucose, 0.6% peptone, 0.3% yeast extract, 0.2% sodium chloride, KH 2 PO 4 0.1%, the rest is water. Adjust the pH to 6.7.

[0033] Under the condition of 30±1℃ and shaking, cultivate 250ml seed bottles for 18 hours, and then insert D into 1000ml fermentation bottles according to 2% volume ratio. 1 Bacterial solution and 2.5% volume ratio seed quantity were inserted into S 4 Bacterial liquid: Under the condition of 33±1°C and shaking, cultivate 1000ml fermentation bottle for 18 hours, measure OD600nm value, and obtain fermentation bacterial agent.

Embodiment 3

[0035] Strain culture: take the above S 4 1 bacterial tube and D of divisional application 1 4 bacterial tubes were respectively inserted into 500ml seed bottles filled with culture medium, which consisted of: 0.85% glucose, 0.7% peptone, 0.4% yeast extract, 0.3% sodium chloride, KH 2 PO 4 0.2%, the rest is water. Adjust the pH to 7.5.

[0036] Incubate the 500ml seed bottle for 16 hours at 35±1°C with shaking, and the D 1 Bacteria and S 4 After the bacterial liquid is mixed, according to the 5% volume ratio of the species, it is then connected to a 100L automatic fermenter for expanded cultivation. The pressure of the fermenter is controlled at 0.5mpa, the tank temperature is 28°C, the ventilation rate is 0.4 (v / v / min), and the stirring speed is 200rpm , cultured for 18 hours to obtain a fermentation broth.

[0037] The above-mentioned fermentation culture was concentrated 4 times by membrane filtration, and the liquid bacterial agent was detected.

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Abstract

The invention provides bacillus amyloliquefaciens S4 for fermentation of a tobacco straw bio-organic fertilizer. The bacillus amyloliquefaciens S4 have a preservation number of CCTCC No: M2010249, are separated and selected from tobacco straws under the conditions of a high temperature and a high nicotine concentration, and can survive in the presence of nicotine. After culture and expanding propagation, the bacillus amyloliquefaciens S4 are added into a waste tobacco straw mixture and the waste tobacco straw mixture with the bacillus amyloliquefaciens S4 is subjected to solid state fermentation so that the waste tobacco straw mixture is transformed into a tobacco straw bio-organic fertilizer. The tobacco straw bio-organic fertilizer can replace other fertilizers to realize tobacco production, can improve a tobacco leaf yield, a tobacco leaf output value and tobacco leaf smoking quality, and can eliminate the pollution produced by tobacco straw waste on the environment.

Description

[0001] This application is a divisional application of the patent application titled "A Preparation Method for Fermentation Strains of Biological Organic Fertilizer", with the application number 201010538124.7 and the filing date 20101027. technical field [0002] The invention relates to the production technology of agricultural bio-organic fertilizer, in particular to a method for preparing strains of tobacco stalk bio-organic fertilizer by solid-state fermentation. Background technique [0003] Tobacco is an important economic crop and plays a very important role in the national economy. In the process of tobacco planting and production in my country, chemical fertilizers have been used for a long time, resulting in a sharp decrease in the amount of soil organic matter, a decrease in the number and quality of aggregates, and degradation of air permeability, which affects the yield and quality of tobacco leaf production. In addition, after the tobacco leaves are harvested,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 高勇陈家任杨树祖秉桥李锡宏
Owner 中国烟草总公司湖北省公司
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