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Glucose responsive gold nanoparticle and preparation method and application thereof

A technology of gold nanoparticles and glucose, which is applied in the fields of chemistry and material science, can solve problems such as interference of detection results and achieve the effect of avoiding interference

Inactive Publication Date: 2013-03-13
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the existing methods are based on oxidation-reduction reactions, reducing substances that may exist in body fluids (such as ascorbic acid in urine, etc.) are likely to interfere with the test results

Method used

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  • Glucose responsive gold nanoparticle and preparation method and application thereof
  • Glucose responsive gold nanoparticle and preparation method and application thereof
  • Glucose responsive gold nanoparticle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example describes the method in detail by modifying gold nanoparticles with isopropylacrylamide-acryloylthioureidoaminophenylboronic acid copolymer and demonstrating its response process to glucose molecules:

[0035] (1) Synthesis of gold nanoparticles (AuNPs): 1 g of chloroauric acid powder was dissolved in 100 mL of ultrapure water to obtain a 1% chloroauric acid stock solution. Add 1 mL of chloroauric acid stock solution to 100 mL of ultrapure water, stir and heat to boiling. 1 mL of trisodium citrate solution (1 mmol) was quickly added, and the solution turned red after a few minutes. Continue heating and stirring for about 15 minutes, cool to room temperature, and store at 4°C for later use to obtain a solution of gold nanoparticles coated with sodium citrate.

[0036] (2) Synthesis of acryloylthioureidoaminophenylboronic acid: Weigh 0.727 g of potassium thiocyanate, dissolve it in 15 mL of anhydrous acetone, add 0.678 g of acryloyl chloride (7.5 mmol), and ...

Embodiment 2

[0049] This example describes the method in detail by modifying gold nanoparticles with isopropylacrylamide-acryloylthioureidoaminophenylboronic acid copolymer and demonstrating its response process to glucose molecules:

[0050] (1) Synthesis of gold nanoparticles (AuNPs): 1 g of chloroauric acid powder was dissolved in 100 mL of ultrapure water to obtain a 1% chloroauric acid stock solution. Add 1 mL of chloroauric acid stock solution to 100 mL of ultrapure water, stir and heat to boiling. 1 mL of trisodium citrate solution (1 mmol) was quickly added, and the solution turned red after a few minutes. Continue heating and stirring for about 15 minutes, cool to room temperature, and store at 4°C for later use to obtain a solution of gold nanoparticles coated with sodium citrate.

[0051] (2) Synthesis of acryloylthioureidoaminophenylboronic acid: Weigh 0.727 g of potassium thiocyanate, dissolve it in 15 mL of anhydrous acetone, add 0.678 g of acryloyl chloride (7.5 mmol), and ...

Embodiment 3

[0057] (1) Synthesis of gold nanoparticles (AuNPs): 1 g of chloroauric acid powder was dissolved in 100 mL of ultrapure water to obtain a 1% chloroauric acid stock solution. Add 1 mL of chloroauric acid stock solution to 100 mL of ultrapure water, stir and heat to boiling. 1 mL of trisodium citrate solution (1 mmol) was quickly added, and the solution turned red after a few minutes. Continue heating and stirring for about 15 minutes, cool to room temperature, and store at 4°C for later use to obtain a solution of gold nanoparticles coated with sodium citrate.

[0058] (2) Synthesis of acryloylthioureidoaminophenylboronic acid: Weigh 0.727 g of potassium thiocyanate, dissolve it in 15 mL of anhydrous acetone, add 0.678 g of acryloyl chloride (7.5 mmol), and stir overnight at room temperature. The reaction solution was filtered, and the yellow supernatant was taken for later use. Weigh 1.0 g of 3-aminophenyl borate hydrochloride (5.77 mmol) and dissolve it in 40 mL of acetone / ...

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Abstract

The invention discloses a glucose responsive gold nanoparticle and a preparation method and an application thereof. The method adopts the reversible addition-fragmentation chain transfer polymerization method to prepare isopropyl acrylamide-acrylyl sulfourea amino phenylboronic acid copolymer that which is modified to the surface of the gold nanoparticle to obtain the gold nanoparticle covered by the copolymer. The gold nanoparticle obtained through the method in disclosed by the invention can generate response with the glucose molecule in a solution; the developing system dispersed by the gold nanoparticle is driven through by the glucose molecule to change the colour of the gold nanoparticle solution at high temperature, so as to realize visual identification and detection for the glucose. The method in disclosed by the invention can be widely used for the field of nanometre bioanalysis.

Description

technical field [0001] The invention relates to a glucose-responsive gold nanoparticle and its preparation method and application, belonging to the fields of chemistry and material science. Background technique [0002] The concentration of glucose in blood and urine is an important clinical indicator of diabetes. According to the World Health Organization (WHO), more than 150 million people worldwide suffered from diabetes in 2004, and by 2030, this number will rise to 366 million. After years of research, a variety of glucose detection methods have been developed. Since most of the existing methods are based on oxidation-reduction reactions, reducing substances that may exist in body fluids (such as ascorbic acid in urine, etc.) are likely to interfere with the test results. Therefore, the development of new rapid glucose detection methods is still of great significance for the treatment and monitoring of diabetes. [0003] In recent years, the rapid development of nano...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/02B82Y30/00B82Y40/00G01N21/78C08F220/54C08F230/06
Inventor 孙涛垒张明曦卿光焱
Owner WUHAN UNIV OF TECH
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