Auxiliary extraction method of biological enzyme for improving dissolution rate of dendrobe bioactive polysaccharide
A bioactive polysaccharide and auxiliary extraction technology, which is used in drug combinations, allergic diseases, antidote, etc., can solve problems such as amplifying the content of polysaccharides, and achieve the effects of improving product quality and yield, improving purity, and reducing protein content.
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[0038] Example
[0039] A biological enzyme-assisted extraction method for improving the dissolution rate of Dendrobium bioactive polysaccharides:
[0040] (1) Crush the dried wild Dendrobium Huoshan or Dendrobium officinale (Huoshan where it is produced) and pass it through a 40-mesh sieve, weigh 50g and add 500mL 95% edible ethanol Soxhlet to reflux for 1h;
[0041] (2) Dry the above-mentioned defatted product into dry powder. According to the mass fraction, take 5g of Dendrobium dry powder and soak it in 100mL of distilled water and adjust the pH to 7.0, then heat the water bath to 50℃, and then add 1.5% of the mass of Dendrobium dry powder. Neutral cellulase, 2.0% xylanase and 1.5% pectinase, enzymatically decompose and destroy cellulose, hemicellulose and pectin in cell tissues for 2 hours;
[0042] (3) Continue to add neutral protease equivalent to 2% of the mass of Dendrobium dry powder to the soaking solution at 50°C and pH 7.0, and keep it for 2 hours to decompose and destroy...
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