Auxiliary extraction method of biological enzyme for improving dissolution rate of dendrobe bioactive polysaccharide

Abstract

The invention discloses an auxiliary extraction method of biological enzyme for improving the dissolution rate of dendrobe bioactive polysaccharide, which is used for auxiliary extracting of the dendrobe polysaccharide by various biological enzymes of which the most proper pH is close to be neutral in a compounding way step by step. The auxiliary extraction method comprises the following steps of: firstly selecting cellulose, xylanase and pectinase to decompose substances of dendrobe cell wall, i.e. cellulose, hemicellulose, pectin substance, decomposing protein and polypeptide in cells by proteasem, so that the polysaccharide can be sufficiently dissolved in water, and carrying out HPSEC (high performance size exclusion chromatography) separation, parallax and refraction detector detection, and a phenol-sulfuric acid method on a final product to detect the content of reducing sugar. According to the auxiliary extraction method, the dissolution rate of dendrobe bioactive polysaccharide can be effectively improved, the protein content can be obviously reduced, the product quality and the yield can be greatly improved, and the use and the discharge of the acid-base solution and the toxic organic reagent and the generation of the microwave radiation can be avoided in the production technology, so that the auxiliary extraction method is more energy-saving and environment-friendly.

Description

technical field [0001] The invention relates to a method for extracting dendrobium polysaccharides, in particular to a biological enzyme-assisted extraction method for improving the dissolution rate of dendrobium bioactive polysaccharides. Background technique [0002] 1. Medicinal value of medicinal Dendrobium and its polysaccharides [0003] The wild Huoshan Dendrobium, known internationally as the "Giant Panda in the Medicine World", also known as Mi Hu, has a history of more than 200 years. Originally produced in Huoshan, Anhui, my country and other places, it is the first protected variety among more than 200 endangered and scarce varieties in the national major modern Chinese medicine project. It is the best product among medicinal Dendrobium, and it is also a rare and precious Chinese herbal medicine unique to Huoshan County with geographical indications of origin. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have records. The polysaccharide...

AI Technical Summary

Problems solved by technology

In the polysaccharide extraction process of the traditional water extraction and alcohol precipitation method, the decomposition of insoluble polysaccharides such as cellulose and hemicellulose basically does not occur, so the extracted product does not contain monosaccharides and oligosaccharides produced by the decomposition of cellulose and hemicellulose chain, the above detection method is suitable; while the enzyme technology decomposes cellulose and hemicellulose during the extraction process to produce an uncertain amount of reducing monosaccharides and oligosaccharides, the above detection method is actually used to determine the total sugar in the extract content, the total sugar content is regarded as the polysaccharide content, which actually amplifies the polysaccharide content. Therefore, any patent that uses enzyme technology to extract polysaccharides from Dendrobium dendrobium claims to increase the content of polysaccharides when using the above detection method (or without explicitly mentioning the detection method) Yes, it's all unbelievable

Images