Sec-butyl alcohol tolerant bacterial strain as well as screening method and application thereof

Abstract

The invention relates to a sec-butyl alcohol tolerant bacterial strain named as S-2, wherein the class name of the sec-butyl alcohol tolerant bacterial strain is Lysinibacillus fusiformis, the preservation number is CGMCC No. 6769, the preservation date is November 2, 2012, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, and the preservation unit is China General Microbiological Culture Collection Center. The sec-butyl alcohol tolerant bacterial strain provided by the invention is high in sec-butyl alcohol tolerance and can be applied to establishing a sec-butyl alcohol engineering strain through the metabolic engineering and the genetic engineering, thereby producing sec-butyl alcohol by the microbiological fermentation method; therefore, the shortcomings that the evidence of sec-butyl alcohol synthesis gene existing in the bacteria and the evidence in the biochemistry aspect are still not found at present and that the sec-butyl alcohol synthesis path is not found in the microorganism in nature are made up; simultaneously, the sec-butyl alcohol tolerant bacterial strain has excellent sec-butyl alcohol biodegrability; and therefore, the sec-butyl alcohol tolerant bacterial strain also can be applied to bioremediation of sec-butyl alcohol pollution.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to sec-butanol-tolerant bacterial strains, in particular to a sec-butanol-tolerant bacterial strain and a screening method and application thereof. Background technique [0002] Second-butanol has many uses, and can be used as an industrial solvent, emulsifier, dye, dispersant, dehydrating agent, paint remover and industrial detergent. In addition, sec-butanol can also be used as a component to increase the octane number of gasoline, a plasticizer, a beneficiation agent and a herbicide. [0003] The large-scale industrial production of sec-butanol mainly depends on the hydration of 1-butene and 2-butene. At present, the output of sec-butanol reaches 900,000 tons / year, and the manufacturers are mainly concentrated in Europe and the United States. [0004] Second-butanol cannot be produced directly by fermentation, and the production method is mainly chemical method: first, the carbo...

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Problems solved by technology

However, there is still no genetic and biochemical evidence for sec-butanol synthesis in bacteria, and no biosynthesis pathway for sec-butanol has been found in natural microorganisms

[0006] Through the search, no patent publications related to this patent application were found

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