Formula of anabaena culture medium
A culture medium, technology of Anabaena, applied in the field of medium formula of Anabaena
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Embodiment 1
[0010] Prepare the culture medium with the following formula:
[0011] NH 4 NO 3 200mg / L, NaNO 3 500mg / L, K 2 SO 4 400mg / L, CaCl 2 .2H 2 O 150mg / L, MgSO 4 .7H 2 O 100mg / L, K 2 HPO 4 400mg / L, Na 2 S 2 o 3 .5H 2 0 15mg / L,H 3 BO 3 20mg / L, CuSO 4 .5H 2 O 2mg / L, Mo(NO 3 ) 3 .5H 2 O 1.5mg / L;
[0012] Ferric ammonium citrate 0.2mg / L, niacin 0.1mg / L, vitamin B 6 0.2mg / L, vitamin B 1 0.2mg / L, NNA0.05mg / L, IBA0.03mg / L.
[0013] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.
Embodiment 2
[0015] NH 4 NO 3 250mg / L, NaNO 3 550mg / L, K 2 SO 4 500mg / L, CaCl 2 .2H 2 O 200mg / L, MgSO 4 .7H 2 O 150mg / L, K 2 HPO 4 500mg / L, Na 2 S 2 o 3 .5H 2 0 20mg / L,H 3 BO 3 25mg / L, CuSO 4 .5H 2 O 4mg / L, Mo(NO 3 ) 3 .5H 2 O 2mg / L;
[0016] Ferric ammonium citrate 0.4mg / L, niacin 0.2mg / L, vitamin B 6 0.3mg / L, vitamin B 1 0.3mg / L, NNA 0.1mg / L, IBA 0.05mg / L.
[0017] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.
Embodiment 3
[0019] NH 4 N O 3 220mg / L, NaNO 3 520mg / L, K 2 SO 4 450mg / L, CaCl 2 .2H 2 O 170mg / L, MgSO 4 .7H 2 O 120mg / L, K 2 HPO 4 450mg / L, Na 2 S 2 o 3 .5H 2 0 17mg / L,H 3 BO 3 22mg / L, CuSO 4 .5H 2 O 3mg / L, Mo(NO 3 ) 3 .5H 2 O 1.7mg / L;
[0020] Ferric ammonium citrate 0.3mg / L, niacin 0.15mg / L, vitamin B 6 0.25mg / L, vitamin B 1 0.25mg / L, NNA0.07mg / L, IBA0.04mg / L.
[0021] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.
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