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Method for valuing standard substance of C reaction protein

A technology for reacting proteins and standard substances, which is used in measuring devices, material analysis by electromagnetic means, instruments, etc., to achieve accurate results, improve CRP solubility, and improve reliability.

Inactive Publication Date: 2013-03-27
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the actual detection work, the C-reactive protein analyzer is mainly used to measure C-reactive protein, which requires the use of C-reactive protein standard substances, but there are still few studies on whether the standard substance values ​​are accurate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation process of C reaction standard substance:

[0032] (1) Prepare Tris-HCl (pH7.5), 0.35moL / NaCl, 2mmol / L CaCl at a concentration of 20mmol / L 2 Solution, 0.5%NaN 3 Used to dissolve purified CRP.

[0033] (2) Prepare a CRP solution with a concentration of 50 μg / g.

[0034] (3) Dispense the CRP solution into 500 μL cryopreservation tubes, 400 μL solution into each cryopreservation tube, and store the subpackaged standard substances in a refrigerator at -80 degrees Celsius.

[0035] (2) Determination of standard substances:

[0036] (1) Three specific peptides of ESDTSYVSLK, APLTKPLK, ALKYEVQGEVFTK were selected for CRP protein quantification.

[0037] (2) The above three peptides were synthesized, and the three corresponding isotope-labeled peptides were synthesized using all deuterium-labeled leucamino groups at the same time.

[0038] (3) Take the national standard substances of leucine, valine, proline and / or phenylalanine as the standard, and use iso...

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Abstract

The invention provides a method for valuing a standard substance of C reaction protein, wherein C reaction protein (CRP) is found by Tillet and Francis in 1930. CRP is acute-phase protein synthesized by liver cells when an organism is subjected to inflammation stimulation of microorganism invasion or tissue damage and the like. In actual detection work, a C reaction protein analysis meter is mainly adopted to test C reaction protein and needs to use the standard substance of C reaction protein, but research on whether the valuing of the standard substance of the C reaction protein is accurate is still less. The invention aims at filling up the technical blank and provides a method for valuing the standard substance of C reaction protein, which has good accuracy, reliability and traceability.

Description

technical field [0001] The invention relates to a method for determining the value of a standard substance, in particular to a method for determining the value of a C-reactive protein standard substance. Background technique [0002] The measurement of the characteristic values ​​of samples that have passed the uniformity and stability tests is called characterization in the technical field of standard substances (the original text is characterize, that is, the measurement of material characteristics); The quantitative value of the characteristic is confirmed by metrology traceability (hereinafter referred to as traceability) and the rationality of the corresponding measurement uncertainty assessment. , generally referred to as authentication in other technical fields). Therefore, the determination of certified reference materials should actually be divided into two steps: first, measure the characteristic values ​​of the samples that have passed the uniformity and stabilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 宋德伟李红梅徐蓓
Owner NAT INST OF METROLOGY CHINA
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