Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry)
A LA-ICP-MS, isotope dilution technology, applied in the field of in-situ analysis of inorganic mass spectrometry, to achieve the effect of easy implementation, good reproducibility and simple operation
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Embodiment 1
[0023] The sample in the example is a goat brain tissue section sample prepared by the laboratory itself.
[0024] (1) Preparation of goat brain tissue slice samples: 100 g of goat brain tissue samples were mixed and homogenized, frozen at -20°C, and sliced with a thickness of 20 μm using a fully automatic cryostat. The mass can be obtained from the weight change of the slide before and after the sample is placed, and the sample preparation process is as follows: figure 1 . Prepare 10 goat brain tissue slice samples in parallel, select 5 slices for ID-LA-ICP-MS analysis, and take another 5 slice samples prepared in parallel, scrape off the tissue samples with a plastic blade, collect and use Microwave digestion, using isotope dilution HR-ICP-MS to measure the iron content.
[0025] (2) The construction of the "tissue anti-off boundary" of goat brain tissue slice samples and the addition of isotope diluent: after weighing the tissue slices, use an immunohistochemical pen to...
Embodiment 2
[0036] The sample in the example is a bovine liver simulated tissue section sample prepared by the laboratory itself.
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