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Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry)

A LA-ICP-MS, isotope dilution technology, applied in the field of in-situ analysis of inorganic mass spectrometry, to achieve the effect of easy implementation, good reproducibility and simple operation

Active Publication Date: 2014-06-04
NAT INST OF METROLOGY CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still two technical difficulties in the application of this method. One is how to determine the quality of the diluent that reacts with the sample to be tested; the other is how to determine whether the isotope exchange between the diluent and the sample has reached equilibrium.
Therefore, so far, the combination of IDMS method and LA-ICP-MS for in situ, micro-area absolute quantitative analysis of tissue section samples has not been reported.

Method used

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  • Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry)
  • Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry)
  • Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry)

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The sample in the example is a goat brain tissue section sample prepared by the laboratory itself.

[0024] (1) Preparation of goat brain tissue slice samples: 100 g of goat brain tissue samples were mixed and homogenized, frozen at -20°C, and sliced ​​with a thickness of 20 μm using a fully automatic cryostat. The mass can be obtained from the weight change of the slide before and after the sample is placed, and the sample preparation process is as follows: figure 1 . Prepare 10 goat brain tissue slice samples in parallel, select 5 slices for ID-LA-ICP-MS analysis, and take another 5 slice samples prepared in parallel, scrape off the tissue samples with a plastic blade, collect and use Microwave digestion, using isotope dilution HR-ICP-MS to measure the iron content.

[0025] (2) The construction of the "tissue anti-off boundary" of goat brain tissue slice samples and the addition of isotope diluent: after weighing the tissue slices, use an immunohistochemical pen to...

Embodiment 2

[0036] The sample in the example is a bovine liver simulated tissue section sample prepared by the laboratory itself.

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Abstract

The invention discloses a method for in-situ measuring iron content in a biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry). The method comprises the following steps of building a sample boundary by adopting a tissue anti-dropping material for a biological section sample, and adding quantitative isotopic dilution agent in a boundary to adequately exchange isotope of the tissue sample; optimizing the addition way of the isotopic dilution agent, the isotopic balance time and the condition of the in-situ isotopic ratio measurement technology; in-situ measuring the iron content in the uniform tissue section sample of goat brain and beef liver by adopting the isotopic dilution-LA-ICP-MS technology, wherein the measurement result is consistent with that of the microwave digestion-isotopic dilution method. The method can be further applied to the in-situ and microcell quantitative measurement and imaging analysis of metal elements in the biological tissue section sample in clinical process, and an important clinical significance for establishing the connection between the distribution variation of iron element in the tissue sample and the typical neurological disease can be realized.

Description

technical field [0001] The invention relates to the technical field of in-situ analysis of inorganic mass spectrometry, in particular to a method for in-situ determination of iron content in biological tissue samples by isotope dilution LA-ICP-MS. Background technique [0002] Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a new technology for solid micro-area analysis based on mass spectrometry detection combined with laser ablation sampling technology. This technology has relatively simple pretreatment for solid sample injection, and the dry aerosol introduced into the plasma has less interference than wet sample injection, and its in-situ (in-situ), micro-area, rapid analysis advantages, and high sensitivity and low detection The ability to measure the content of various elements and isotopic compositions has become a promising analytical method in biomedical research, and has become the frontier of mass spectrometry in recent years. In the ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/38G01N27/626
Inventor 冯流星王军张丹
Owner NAT INST OF METROLOGY CHINA
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