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Protease preparation prepared by using sea cucumber cooking liquor and preparation method thereof

The technology of protease preparation and sea cucumber cooking liquid is applied in the field of protease preparation, which can solve the problems of destroying the ecological environment, soil salinization, waste of resources and the like, and achieves the effects of no toxic side effects and broad application prospects.

Inactive Publication Date: 2013-04-03
QINGDAO AIHUALONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, these waste liquids are directly discharged and discarded, but the waste liquid contains a large amount of nutrients, and its salt content is as high as 10% to 15%, which not only causes waste of resources, but also leads to soil salinization and damage to the ecological environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A protease preparation prepared by using sea cucumber cooking liquid and a preparation method thereof, the preparation method comprising the following steps:

[0024] (1) Ingredients: Use desalted sea cucumber cooking liquid dry powder as the main raw material of the medium, add an equal amount of bean dregs, and then add inorganic salts in an amount of 0.5% of the total dry weight of the medium, add water and mix well to make the water content 50% culture medium;

[0025] (2) High temperature sterilization: sterilize at 121°C for 20 minutes;

[0026] (3) Inoculation: After the culture medium is cooled to room temperature, inoculate the strains, and the inoculum amount is 10% of the total weight of the culture medium and mixed evenly to form a fermentation material;

[0027] (4) Fermentation: The fermented material is fermented at 35°C until it matures to obtain the protease preparation; the activity of acidic protease is ≥2500U / g dry basis.

[0028] (5) Drying: the p...

Embodiment 2

[0031] A protease preparation prepared by using sea cucumber cooking liquid and a preparation method thereof, the preparation method comprising the following steps:

[0032] (1) Ingredients: take the desalted sea cucumber cooking liquid dry powder as the main raw material of the medium, add an equal amount of bran, and then add 0.5% inorganic salts of the total dry weight of the medium, add water and mix well to make a water content of 50 % of medium;

[0033] (2) High temperature sterilization: sterilize at 121°C for 20 minutes;

[0034] (3) Inoculation: After the culture medium is cooled to room temperature, inoculate the strains, and the inoculum amount is 10% of the total weight of the culture medium and mixed evenly to form a fermentation material;

[0035] (4) Fermentation: ferment the fermented material at 37°C until it matures to obtain the protease preparation;

[0036] (5) Drying: the protease preparation is placed in the Quchi, and then water vapor is introduced i...

Embodiment 3

[0039] A protease preparation prepared by using sea cucumber cooking liquid and a preparation method thereof, the preparation method comprising the following steps:

[0040] (1) Ingredients: Desalted sea cucumber cooking liquid dry powder is used as the main raw material of the medium, and bean dregs and bran are added. The mass ratio of the above three substances is 2:1:1, and the amount of addition is 0.5 of the total dry weight of the medium. % inorganic salts, add water and mix to make a medium with a water content of 50%;

[0041] (2) High temperature sterilization: sterilize at 121°C for 20 minutes;

[0042] (3) Inoculation: After the culture medium is cooled to room temperature, inoculate the strains, and the inoculum amount is 10% of the total weight of the culture medium and mixed evenly to form a fermentation material;

[0043] (4) Fermentation: ferment the fermented material at 37°C until it matures to obtain the protease preparation;

[0044] (5) Drying: the prot...

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PUM

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Abstract

The invention discloses a protease preparation prepared by using sea cucumber cooking liquor, which is prepared through the following steps of: (1) material preparation: through taking desalted sea cucumber cooking liquor dry powder as a main raw material, adding water into the dry powder uniformly so as to prepare a culture medium; (2) high-temperature sterilization: carrying out sterilization on the culture medium for 20 minutes at a temperature of 121 DEG C; (3) inoculation: inoculating strains in the culture medium subjected to sterilization, and uniformly mixing so as to obtain a fermentation material; and (4) fermentation: fermenting the fermentation material at a temperature of 36-38 DEG C until the fermentation material is mature, thereby obtaining the protease preparation. The protease preparation is higher and stable in acid protease activity, and is suitable for being used as an animal feed. The invention also discloses a preparation method of the protease preparation.

Description

technical field [0001] The invention relates to a protease preparation, in particular to a protease preparation prepared by using sea cucumber cooking liquid, and also relates to a preparation method of the protease preparation. Background technique [0002] my country's annual pig production ranks first in the world, and it is also the country with the largest amount of pig breeding. Pigs need high energy and protein in the process of growth and development, but the digestive enzymes secreted by the digestive organs of pigs are insufficient, and the digestion ability is poor, so the utilization rate of feed is low and the consumption is large, so the potential for using protease preparations is great. [0003] At present, protease preparations are all produced by microbial fermentation, and the biggest disadvantage is poor protease stability, which is mainly caused by the existing production process of protease preparations. [0004] Sea cucumber cooking liquid is the wast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/62A23K1/165C12R1/685C12R1/69
Inventor 荆晓丽
Owner QINGDAO AIHUALONG BIOTECH