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Application of 4,5-dimethoxyphthalaldehyde in detection of ammonium nitrogen in water and determination method

A technology of dimethoxy-phthalaldehyde and ammonium nitrogen, which is applied in the detection field of ammonium nitrogen content in water, can solve the problems of slow fluorescence reaction rate, difficult and low-cost portable ammonium nitrogen and the like

Inactive Publication Date: 2013-04-03
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

However, the fluorescence reaction rate of o-phthalaldehyde and ammonium nitrogen is slow, and it takes 180 minutes to reach equilibrium (Yu Xiangxiang, Guo Weidong, 2007. High-sensitivity fluorescence method for the determination of low-level ammonium nitrogen in seawater. Ocean Science 31(4), 37 -41); In addition, most of the existing portable optical instruments use LED as the light source, and the wavelength of common LED lamps is mostly above 370 nm. Therefore, it is difficult to develop a low-cost portable ammonium nitrogen fluorescence detector based on the OPA method

Method used

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  • Application of 4,5-dimethoxyphthalaldehyde in detection of ammonium nitrogen in water and determination method
  • Application of 4,5-dimethoxyphthalaldehyde in detection of ammonium nitrogen in water and determination method
  • Application of 4,5-dimethoxyphthalaldehyde in detection of ammonium nitrogen in water and determination method

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Embodiment 1

[0133] 1) Make a working curve:

[0134] According to 1.3.2, take 20 mL of ammonium nitrogen working solution with the ammonium nitrogen concentration of 0 μmol / L, 0.250 μmol / L, 0.500 μmol / L, 0.750 μmol / L, 1.000 μmol / L, each part of ammonium nitrogen working solution Add 2.5 mL of 1 mol / L EDTA-4Na solution, 0.625 mL of 1.8 g / L M 2 OPA solution (MO in the reaction solution 2 PA concentration is 0.045 g / L), 1.88 mL 1.26 g / L Na 2 SO 3 solution (the concentration of sodium sulfite in the reaction solution is 0.095 g / L), and 1 mol / L NaOH solution to adjust the pH of the system to about 11.7 (the volume of NaOH solution added is about 0.2 mL); after reacting for 60 min at room temperature, use RF-5301 PC-type fluorescence spectrophotometer (both excitation and emission slit widths are set to 5 nm during measurement), the excitation wavelength is set to 379 nm, the emission wavelength is 482 nm, and the fluorescence intensity IF is measured, with IF as the ordinate, the ammonium i...

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Abstract

The invention discloses application of 4,5-dimethoxyphthalaldehyde in detection of ammonium nitrogen in water and a determination method. The determination method comprises the following concrete steps: 1) successively adding an EDTA-4Na solution, a 4,5-dimethoxyphthalaldehyde solution and a sodium hydrosulphite solution into ammonium nitrogen working fluid, then adjusting the pH value of an obtained system by using a sodium hydroxide solution, determining fluorescence curves in a range of 400 to 600 nm when lambda ex is equal to 379 nm after reaction equilibrium, recording fluorescence intensity at a maximum emission wavelength and drafting a working curve; and 2) substituting fluorescence intensity of a sample solution at a maximum emission wavelength in the working curve to quantify the content of ammonium nitrogen in the sample solution. According to the method provided by the invention, reaction equilibrium time for 4,5-dimethoxyphthalaldehyde and ammonium nitrogen is shortened to 50 min, red shift of maximum excitation wavelength of a reaction product approaches a visible light zone, so a foundation is laid for development of a portable ammonium nitrogen on-line fluorescence detector.

Description

technical field [0001] The invention relates to the detection field of ammonium nitrogen content in water, in particular to the application of 4,5-dimethoxyphthalaldehyde in the detection of ammonium nitrogen in water, and the use of 4,5-dimethoxyphthalaldehyde as a fluorescent Reagent method for the determination of trace ammonium nitrogen in seawater. Background technique [0002] O-phthaldialdehyde (o-phthaldialdehyde, referred to as OPA) is currently reported as a fluorescent reagent widely used in the detection of ammonium nitrogen in water. In the presence of sodium sulfite, the fluorescent product generated by it and ammonium nitrogen has a maximum excitation wavelength of 361-365 nm. The maximum emission wavelength is 422-425 nm (Genfa, Z., Dasgupta, P., 1989. Fluorometric measurement of aqueous ammonium ion in a flow injection system. Analytical Chemistry 61, 408-412; Amornthammarong, N., Zhang, J.Z., Ortner, P.B., 2011. An autonomous batch analyzer for the determi...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06
Inventor 梁英潘英明黄国保曹毅成
Owner GUILIN UNIV OF ELECTRONIC TECH
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