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Method for preparing resveratrol extract by using microbial fermentation technology

A microbial fermentation and resveratrol technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, environmental pollution, and low extraction rate, so as to avoid emulsification and accelerate The extraction process and the effect of improving the extraction rate

Active Publication Date: 2014-04-16
HUBEI SANXIN BIOTECH
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Problems solved by technology

(2) Resveratrol can be obtained by the above three methods, but the process is complicated, the operation cycle is long, the loss of medicinal materials is large, the cost is high, and the synthesis process is accompanied by by-products and environmental pollution, and the extraction rate is not good. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The method for preparing resveratrol extract by microbial fermentation technology, the specific steps are as follows:

[0016] (1). Crush the knotweed to obtain the knotweed powder; the particle size of the knotweed powder reaches 200-800 mesh;

[0017] (2). Add 1.5 times the weight of water, 4.8‰ of fermentation powder, 6.4‰ of glucoamylase, 2.4‰ of active dry yeast and 2.4‰ of urea into Polygonum cuspidatum powder;

[0018] (3). Mix the above materials thoroughly and ferment at room temperature for 8 days to obtain the resveratrol extract.

Embodiment 2

[0019] Example 2: Dry the resveratrol extract, sample 1g, fix it with 50ml of methanol, and ultrasonicate for 1h; take 1ml of the solution and dilute it with 30% acetonitrile, and then fix it again 10ml; take 1ml of the fixed solution and pass it through Filter the microporous membrane into the centrifuge tube, inject it into the incubator three times (about 30ul each time) for testing, and get the following (extraction rate) data: the first time is 5.37%, the second time is 5.19%, and the third time is 5.37%. The second time was 5.35%. Based on the above data, it can be seen that after fermentation treatment, the average extraction rate of resveratrol in Polygonum cuspidatum is about 5.30%. Conclusion: The use of fermentation technology increases the content of resveratrol in raw materials by 10 to 20 times, effectively increases the content of substrates, and maximizes the economic value and (physiological efficacy) use value of raw materials.

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Abstract

The invention provides a method for preparing resveratrol extract by using a microbial fermentation technology. The method comprises the following steps of: (1) crushing polygonum cuspidatum to obtain polygonum cuspidatum powder; (2) adding water of which the weight is 1 to 2 times that of raw materials, fermentation powder of which the weight is 0.48 percent of that of the raw materials, saccharifying enzyme of which the weight is 0.64 percent of that of the raw materials, active dry yeast of which the weight is 0.24 percent of that of the raw materials and urea of which the weight is 0.24 percent of the raw materials into the polygonum cuspidatum powder; and (3) fully stirring the materials uniformly, and fermenting for 7 to 9 days at normal temperature, thereby obtaining the resveratrol extract. By using the microbial fermentation technology, the resveratrol content of the resveratrol extract is improved by 10 to 20 times, the economic value and the (physiological efficiency) use value of the raw materials are brought into play to the utmost benefit, the emulsification phenomenon in the subsequent extraction procedure is avoided, the extraction progress is accelerated, and the extraction rate of the resveratrol is improved.

Description

technical field [0001] The invention relates to a method for preparing resveratrol extract by microbial fermentation technology. Background technique [0002] At present, many companies mainly use the characteristics of resveratrol to be easily soluble in organic solvents in the extraction process of resveratrol. The following three methods are mainly used in the processing of raw materials: [0003] 1. Reflux extraction method: Weigh a certain weight of Polygonum cuspidatum powder, reflux extract with 4 times, 3 times, 3 times of 95% ethanol for 2 hours, 2 hours, 1 hour, combine the extracts to obtain the pretreatment raw material, namely resveratrol Alcohol extract; after filtration, the filtrate is concentrated under reduced pressure to an extract, dried in vacuum, and weighed to obtain the pretreatment raw material. [0004] 2. Ultrasonic extraction method: Weigh a certain weight of Polygonum cuspidatum powder, soak it in ethanol for 2 hours, put it into an ultrasonic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/22C12R1/865
Inventor 余国军何兆奎张仁平
Owner HUBEI SANXIN BIOTECH
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