Floating exercising seedling and cultivating method of dendrobium officinale tissue culture seedling

A technology of Dendrobium officinale and its cultivation method, which is applied in cultivation, soilless cultivation, botanical equipment and methods, etc., can solve the problems of increasing pesticide residues in Dendrobium candidum, affecting the medicinal value of Dendrobium candidum, and not being able to enter the homes of ordinary people, etc. Achieve the effect of strong resistance, strong applicability and convenient operation

Active Publication Date: 2013-04-17
李奋勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty and cost of artificial cultivation itself, most research institutions (or companies) stay in the experimental research stage and cannot realize commercial (industrial) production, resulting in extremely high prices of Dendrobium candidum, which are only purchased by high-income people and cannot enter the ordinar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Three days after opening the bottle of the tissue culture seedlings, take the seedlings out of the bottle, wash the medium, and disinfect with 2% potassium permanganate water for 20-30 seconds.

[0042] The substrate ratio is: sawdust; mountain base soil; perlite 3.5:3.5:3, PH value 6-6.5, cluster planting, planting 3-5 plants per hole. It was transplanted on October 10, and 6 days later, it was observed that the substrate was moldy. One month later, the survival rate of the transplanted seedlings was 80%, the plant growth was 1.5 cm, and the stem thickness was basically unchanged.

Embodiment 2

[0044] After the tissue culture seedlings are transported, the medium has been washed, and the dishes are disinfected with 2% potassium permanganate water for 2-3 minutes, and the seedlings are disinfected for 20-30 seconds.

[0045] Substrate ratio: sawdust; perlite 7; 3, PH value 6.8-7, transplanted on October 10, observed on November 9, the survival rate of transplanting was 80%, and the plant growth was unchanged.

Embodiment 3

[0047] Substrate ratio: sawdust; mountain base soil; perlite + mica 3.5: 3.5: 3: 0.6 PH value 6.5 -7, transplanted on October 10, observed on November 9, the survival rate of transplanting is 80%, and the plant growth is 1.6 cm, the stem thickness is basically unchanged.

[0048] Comparative test 4

[0049] Substrate ratio: hummus + perlite 2:1, p H value of 6.2-6.5, transplanted on October 10, and observed on November 9, the survival rate of transplanting is 80%, the plant growth is 2cm, and the stem thickness is basically unchanged .

[0050] Comparative test 5

[0051] Substrate ratio: medicine residue: mountain base soil: perlite=5:3:2, PH value 6.9, transplanted on October 10, and observed on November 9, the survival rate of transplanting is 70%, and the plant has no growth.

[0052] Comparative test 6

[0053] Substrate ratio: chaff: mountain base soil: perlite=5:3:2, PH value 7, transplanted on October 10, observed on November 9, the survival rate of transplanting was 68%, an...

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PUM

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Abstract

The invention discloses a floating exercising seedling and cultivating method of dendrobium officinale tissue culture seedling. The floating exercising seedling and cultivating method: 1) building of a floating exercising seedling pool: site selection, pool building and shed erecting; 2) matrix selection and treatment; 3) before and after bottle-outlet management of tissue culture seedling; 4) floating breeding plate and transplanting method; and 5) exercising seedling nutrient solution preparation of the tissue culture seedling. By means of the floating exercising seedling and cultivating method of dendrobium officinale tissue culture seedling, the exercising seedling time of the dendrobium officinale is shortened, plant survival rate is improved, growth of seedling is promoted, the exercising seedling time is reduced to 1.5 months from the conventional 3 months, the plant survival rate is improved to about 99% from the traditional 60-70%, the plant can grow about 4.5-7cm averagely compared with the conventional exercising seedling period which has zero growth, 5-7 stem sections can be added, the stem thickness can grow 0.27-0.52cm averagely, the number of blades is increased by 7-10, the number of roots is increased by 1-7, the length of the roots is increased by 0.12-0.69cm, and the root thickness is increased by 0.14-0.15cm.

Description

technical field [0001] The invention relates to floating seedling hardening of tissue cultured seedlings of Dendrobium candidum and a cultivation method thereof, in particular to the industrial production of floating hardening and cultivation techniques of tissue cultured seedlings of Dendrobium candidum. It belongs to the technical field of floating seedling hardening and cultivation of Chinese herbal medicine. Background technique [0002] Dendrobium officinale ( Dendrobium Candidum Wall.ex Lindl.) is the most rare and valuable species of Dendrobium medicinal plants. At present, the market demand for Dendrobium candidum is very large, but due to the scarcity of natural resources, the maturity of artificial planting technology needs to be improved, and its market supply capacity is relatively weak. [0003] Since the late 1970s in China, relevant research institutions have conducted some explorations on the tissue culture conditions, transplanting factors, survival rate ...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G31/00
Inventor 李奋勇
Owner 李奋勇
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