Effective part of Houttuynia cordata as well as extracting method and application thereof

A technology of effective parts and extraction methods, which is applied in the field of extracting effective parts of the plant Houttuynia cordata to prepare anti-tumor drugs, and achieves the effect of simple extraction method and strong anti-tumor activity

Active Publication Date: 2013-04-24
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no related reports that the volatile oil, ethyl ac

Method used

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  • Effective part of Houttuynia cordata as well as extracting method and application thereof
  • Effective part of Houttuynia cordata as well as extracting method and application thereof
  • Effective part of Houttuynia cordata as well as extracting method and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0015] Example 1

[0016] An effective part of Houttuynia cordata, which is selected from one or a mixture of two or more of Houttuynia cordata volatile oil, ethyl acetate part of Houttuynia cordata and ethanol part of Houttuynia cordata.

[0017] The method for extracting the effective parts of Houttuynia cordata includes the following steps: Take 500g of crushed whole Houttuynia cordata and place it in a flask (a few zeolite can be put in the flask), add 5000ml water, shake and mix, and soak for about 12 hours. , And then steam distill the houttuynia cordata and the soaking liquid (keep boiling for 6 hours), and collect the distillate. After the distillate is allowed to stand for cooling and stratification, take the upper light yellow oil layer (about 0.5ml) to obtain Houttuynia cordata volatile oil; dry the houttuynia cordata residues extracted with water and use 7500ml petroleum ether, 7500ml ethyl acetate and 7500ml 95% ethanol reflux extraction for 3h (that is, after the pet...

Example Embodiment

[0018] Example 2

[0019] An effective part of Houttuynia cordata, which is selected from one or a mixture of two or more of Houttuynia cordata volatile oil, ethyl acetate part of Houttuynia cordata and ethanol part of Houttuynia cordata.

[0020] The method for extracting the effective parts of Houttuynia cordata includes the following steps: take 500g of the crushed whole Houttuynia cordata and place it in a flask (several zeolite can be put in the flask), add 6000ml of water, shake and mix, and soak for about 15 hours , Then steam distill the houttuynia cordata and the soaking liquid (keep boiling for 8 hours), and collect the distillate. After the distillate is allowed to stand for cooling and stratification, take the upper light yellow oil layer (about 0.6ml) to obtain the Houttuynia cordata volatile oil; dry the houttuynia cordata residues extracted with water and use 10000ml petroleum ether, 10000ml ethyl acetate and 10000ml 95% ethanol was refluxed and extracted for 4 hou...

Example Embodiment

[0021] Example 3

[0022] An effective part of Houttuynia cordata, which is selected from one or a mixture of two or more of Houttuynia cordata volatile oil, ethyl acetate part of Houttuynia cordata and ethanol part of Houttuynia cordata.

[0023] The extraction method of the effective parts of Houttuynia cordata includes the following steps: take 500g of crushed whole Houttuynia cordata and place it in a flask (a few zeolite can be put in the flask), add 4000ml of water, shake and mix, and soak for about 10 hours , And then steam distillation of Houttuynia cordata and soaking liquid (keep boiling for 10h), and collect the distillate. After the distillate is allowed to stand for cooling and layering, take the upper light yellow oil layer (about 0.4ml) to obtain the Houttuynia cordata volatile oil; dry the Houttuynia cordata residues extracted with water and use 9000ml petroleum ether, 9000ml ethyl acetate and 9000ml of 95% ethanol was refluxed for 1 hour, and the obtained extract...

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Abstract

The invention relates to an effective part of Houttuynia cordata. The effective part of Houttuynia cordata is selected from one or a mixture of more than two of Houttuynia cordata volatile oil, Houttuynia cordata ethyl acetate extract and Houttuynia cordata ethyl ethanol. The extracting method of the effective part of Houttuynia cordata comprises the following steps of: carrying out steam distillation for Houttuynia cordata soaked by water to collect distillate; standing, cooling and layering the distillate and taking the upper oil layer to obtain the Houttuynia cordata volatile oil; airing or drying Houttuynia cordata extracted by water, and sequentially carrying out reflux extraction by petroleum ether, ethyl acetate and ethanol; and volatilizing a solvent of the extracting liquor to respectively obtain the Houttuynia cordata ethyl acetate extract and Houttuynia cordata ethyl ethanol. The effective part of Houttuynia cordata has a better anti-tumor effect for human hepatoma carcinoma cells HepG2, human breast cancer cells MCF-7, human lung cancer cells A549 and human lymphoma cells Raji, and can be used to prepare anti-tumor medicines.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an extract of effective parts of the plant Houttuynia cordata, an extraction method and its application in the preparation of antitumor drugs. Background technique [0002] Houttuynia cordata Thunb (Houttuynia cordata Thunb) is a plant of the genus Houttuynia cordata, formerly known as "Houttuynia cordata Thunb". Distributed in the south of the Yangtze River Basin, mainly produced in Zhejiang, Jiangsu, Anhui, Fujian, Henan and other provinces. The whole herb with roots is used medicinally, pungent in nature, slightly cold in taste, and enters the lung meridian. It has the functions of clearing heat and detoxifying, eliminating carbuncle and discharging pus, diuresis and treating stranguria. "Broad Spectrum Antibiotics in Traditional Chinese Medicine". "Compendium of Materia Medica" records: "Its leaves have a fishy smell, so it is commonly kno...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61P35/00
Inventor 张壮丽郑立运赵志鸿张小俊王桂芳张长征陈慧平邹敏张艳王宁马方王东阳王庆端江金花王陆巡赵宁
Owner 河南省医药科学研究院
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