Culture medium adopting continuous enrichment culture for mycoplasma gaujseptium and preparation method

A technology for the cultivation of mycoplasma gallisepticum and bacteria enrichment, which is applied in the field of biological products, can solve the problems of large loss in the concentration process, low culture titer of mycoplasma gallisepticum, and increased vaccine production costs, so as to simplify the production process and reduce production costs Effect

Inactive Publication Date: 2013-04-24
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The culture titer of Mycoplasma gallisepticum cultivated by the above-mentioned traditional culture medium and culture process is low (the concentration of the culture solution is low), and the bacteria solution (semi-finished product) used for seedling production cannot be used directly in the production of inactivated vaccines, and th

Method used

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  • Culture medium adopting continuous enrichment culture for mycoplasma gaujseptium and preparation method
  • Culture medium adopting continuous enrichment culture for mycoplasma gaujseptium and preparation method
  • Culture medium adopting continuous enrichment culture for mycoplasma gaujseptium and preparation method

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Embodiment 1

[0014] The preparation of embodiment 1 culture medium of the present invention

[0015] 1. Continuous enrichment culture medium for Mycoplasma gallisepticum

[0016]

[0017] When cultivating, add 200ml pig serum and 1000 units / ml penicillin, adjust the pH to 7.6-7.8 with 20% NaOH

[0018] 2. The above-mentioned continuous enrichment culture culture medium for Mycoplasma gallisepticum is prepared according to the following steps:

[0019] (1) Inoculate the seeds for the production of Mycoplasma gallisepticum into the Mycoplasma gallisepticum culture medium at a ratio of 1:10, cultivate them at 36-37°C for 18-24 hours, and harvest them; The ratio of 10 was reinoculated and passaged once;

[0020] (2) Inoculate the above-mentioned subcultured bacterial solution into the continuous enrichment medium of the present invention at a ratio of 1:10, cultivate at 37°C, and wait for the pH to drop to about 6.5, add the continuous enrichment culture medium for Mycoplasma gallisepticu...

Embodiment 2

[0021] The preparation of embodiment 2 medium of the present invention

[0022] 1. Continuous enrichment culture medium for Mycoplasma gallisepticum

[0023]

[0024] During cultivation, add 180ml pig serum and 1000 units / ml penicillin, and adjust the pH to 7.6-7.8 with 20% NaOH.

[0025] 2. The above-mentioned continuous enrichment culture culture medium for Mycoplasma gallisepticum is prepared according to the following steps:

[0026] (1) Inoculate the seeds for the production of Mycoplasma gallisepticum into the Mycoplasma gallisepticum culture medium at a ratio of 1:10, cultivate them at 36-37°C for 18-24 hours, and harvest them; The ratio of 10 was reinoculated and passaged once;

[0027] (2) Inoculate the above-mentioned subcultured bacterial solution into the continuous enrichment medium of the present invention at a ratio of 1:10, cultivate at 37° C., and when the pH drops to about 6.5, add the continuous enrichment culture medium for Mycoplasma gallisepticum to ...

Embodiment 3

[0028] The preparation of embodiment 3 medium of the present invention

[0029] 1. Continuous enrichment culture medium for Mycoplasma gallisepticum

[0030]

[0031] During cultivation, add 1800ml pig serum and 1000 units / ml penicillin, and adjust the pH to 7.6-7.8 with 20% NaOH.

[0032] 2. The above-mentioned continuous enrichment culture culture medium for Mycoplasma gallisepticum is prepared according to the following steps:

[0033] (1) Inoculate the seeds for the production of Mycoplasma gallisepticum into the Mycoplasma gallisepticum culture medium at a ratio of 1:10, cultivate them at 36-37°C for 18-24 hours, and harvest them; The ratio of 10 was reinoculated and passaged once;

[0034] (2) Inoculate the above-mentioned subcultured bacterial solution into the continuous enrichment medium of the present invention at a ratio of 1:10, cultivate at 37°C, and wait for the pH to drop to about 6.5, add the continuous enrichment culture medium for Mycoplasma gallisepticu...

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Abstract

The invention relates to a culture medium adopting continuous enrichment culture for mycoplasma gaujseptium and a preparation method, belonging to the technical field of veterinary biology. The culture medium comprises PPLO (pleuropneumonia-like organism), yeast extract powder, glucose and the like, wherein before use, healthy pig serum is added. The culture medium can ensure the high titer and stability of the growth of a semi-finished product, and the semi-finished product bacterium solution prepared by the method disclosed by the invention is up to 10<12> CCU/ml; simultaneously, the culture medium is used for culturing mycoplasma gaujseptium by adopting a continuous enrichment culture process, so that the stable growth phase of the propagated thalli of mycoplasma gaujseptium is prolonged, the active ingredients in the culture medium are furthest utilized and consumed, and then the active antigens of mycoplasma gaujseptium are greatly increased and incremented; and the final bacterium solution can be used for preparing an inactivated vaccine directly or after being diluted, and does not need to be concentrated, so that the production process is simplified, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of biological products, and relates to an improved medium for enriching mycoplasma gallisepticum, in particular to a process for enriching and cultivating mycoplasma gallisepticum. Background technique [0002] Mycoplasma galliseptium (MG) is the pathogen of chicken chronic respiratory disease (CRD), and the infection rate is very high. CRD can be transmitted horizontally and vertically and can persist and spread in flocks for a long time. After chicken farms are infected with MG, the weak chick rate of chicks increases, and the egg production rate of laying hens, broiler body weight and feed conversion rate all decrease, causing serious harm to the development of the chicken industry. At present, the treatment of Mycoplasma gallisepticum in various chicken farms mainly uses antibiotics, but Mycoplasma gallisepticum is prone to drug resistance, and the effect is not satisfactory. Immunization vaccine is th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
Inventor 不公告发明人
Owner 兆丰华生物科技(南京)有限公司
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