Gene marker for detecting cancer tissue and para-carcinoma tissue of primary liver cancer

A gene chip, specific technology, applied in the field of medicine

Inactive Publication Date: 2013-04-24
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, so far, there is little understanding of the genes closely related to primary liver cancer in this field, so there is an urgent need in this field to further separate various genes closely related to primary liver cancer, and to find out the genes that are useful for diagnosing the occurrence, metastasis, and development of liver cancer. Genetic markers associated with relapse or detection

Method used

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  • Gene marker for detecting cancer tissue and para-carcinoma tissue of primary liver cancer
  • Gene marker for detecting cancer tissue and para-carcinoma tissue of primary liver cancer
  • Gene marker for detecting cancer tissue and para-carcinoma tissue of primary liver cancer

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preparation example Construction

[0034] The preparation of the gene chip of the present invention can adopt the conventional manufacturing method of biochip. For example, if the solid phase carrier is a modified glass slide or a silicon wafer, and the 5' end of the probe contains amino-modified poly dT strings, the oligonucleotide probe can be formulated into a solution, and then the spotting instrument will spot it on The gene chip of the present invention can be obtained by modifying glass slides or silicon slices, arranging them into a predetermined sequence or array, and then fixing them by placing them overnight. If the oligonucleotide probe does not contain amino modification, its preparation method can also refer to: "Gene Diagnosis Technology-Non-radioactive Operation Manual" edited by Wang Shenwu; J.L.erisi, V.R.Iyer, P.O.BROWN.Exploring the metabolic and genetic control of gene expression on a genomic scale. Science. 1997; 278: 680 and Ma Liren, edited by Jiang Zhonghua. Biochip. Beijing: Chemical I...

Embodiment 1

[0051] Embodiment 1, the preparation of RNA sample

[0052] Tissue sample:

[0053] Forty-nine pairs of cancer and paracancerous tissues were obtained from surgical resection specimens of patients with primary liver cancer, and these specimens were obtained from Qidong Liver Cancer Institute and the First Affiliated Hospital of Zhejiang University. 10 cases of normal liver from accidental death. All the above-mentioned specimens were obtained with the approval of the ethics committee of the WHO partner organization authorized by the Shanghai Municipal Government. The clinical data of tissue samples include: gender, age, tumor size, pathological grade (Edmonson), liver cirrhosis, metastasis, recurrence, HBV and HCV infection, etc. All patients did not receive chemotherapy before operation, and the postoperative follow-up was up to 60 months.

[0054] Gene chip:

[0055] Whole-genome expression profile chip, using the crystal core of Capital Bio Co., Ltd. (http: / / www.capital...

Embodiment 2

[0061] Example 2, screening genes with significant differences

[0062] 49 cases of cancer samples and normal samples (10 cases mixed), 49 cases of paracancerous samples and normal samples (10 cases mixed) were labeled with two fluorescent dyes, cy3 and cy5, respectively, and competitively hybridized with the probes on the genome-wide expression profile chip . Chip core after hybridization LuxScan TM The 10K microarray chip scanner scans to obtain the result image, and then quantifies the hybridization result through the LuxScan 3.0 general microarray image analysis software that comes with it. Thus, the expression profile data of 49 pairs of cancer samples to normal samples (cancer / normal, Cancer / Normal, C / N) and para-cancer samples to normal samples (pre-cancer / normal, Pericancerous / Normal, P / N) were obtained.

[0063] The above 49 cancer / normal microarray results and 49 paracancerous / normal microarray results were first normalized by LOWESS (local weighted regression a...

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Abstract

The invention relates to a gene marker for detecting a cancer tissue and a para-carcinoma tissue of primary liver cancer. The inventor firstly screens out 33 specific genes by virtue of a statistical method by analyzing a whole genome expression profile of the cancer tissue and the para-carcinoma tissue of a patient suffering from the primary liver cancer. The test proves that the specific markers can be very effectively used for distinguishing the cancer tissue and para-carcinoma tissue of the patient suffering from the primary liver cancer.

Description

technical field [0001] The invention belongs to the field of medicine; more specifically, the invention relates to gene markers for detecting primary liver cancer cancer tissue and adjacent tissue. Background technique [0002] The occurrence and development of anything is under the joint action of internal and external factors, internal factors are dominant, and external factors are supplementary. As the genetic medium of life, genes are the basic internal causes in the birth, aging, disease and death of organisms. Most genes perform biological functions by transcribing ribonucleic acid mRNA and then translating to protein. Although the function of the gene is mainly reflected by the protein, it can still be partially reflected by the mRNA content of the gene transcription product. [0003] The genome-wide expression profile chip contains more than 20,000 probes, and uses the principle of DNA double-strand homologous complementarity to detect the content of various mRNAs ...

Claims

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Application Information

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IPC IPC(8): C12N15/60C12N15/54C12N15/53C12N15/52C12N15/12C12Q1/68C12N15/11C40B40/06
Inventor 何祥火魏霖梁琳慧顾健人
Owner SHANGHAI INST OF ONCOLOGY
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