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Method for measuring contents of chromium, nickel, arsenic, selenium, cadmium, mercury and lead in urine

A determination method and technology of lead content, applied in the field of urine collection, analysis and detection, and determination of trace elements in urine

Active Publication Date: 2013-04-24
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Current research on the effect of smoking on heavy metal levels in smokers has not been reported
Atomic Absorption Spectroscopy Cannot Quickly and Accurately Detect Trace Heavy Metal Elements in the Urine of Various Smokers

Method used

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  • Method for measuring contents of chromium, nickel, arsenic, selenium, cadmium, mercury and lead in urine
  • Method for measuring contents of chromium, nickel, arsenic, selenium, cadmium, mercury and lead in urine

Examples

Experimental program
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Effect test

example 1

[0058] 1. Instruments and reagents: Inductively coupled plasma: Agilent, 7500A inductively coupled plasma mass spectrometer; Mars 5 microwave digestion instrument (CEM, USA), equipped with PTFE high-pressure closed digestion tank; electronic balance, CP225D, with a sensitivity of 0.0001g (Sartorius AG company); argon: 99.99% pure;

[0059] Ultra-pure water. Concentrated nitric acid with a mass percentage of 65% and hydrogen peroxide with a mass percentage of 30% are chromatographically pure; mixed standard and mercury standard (10.0 mg / L, Agilent, USA).

[0060] 2. Sample treatment: Take 10 mL of urine in a polypropylene bucket, put it into a microwave digestion tank, and then put it into a microwave digestion apparatus for microwave digestion. (The maximum liquid in the digestion tank is ?) Put 1 mL of 30% (v / v) hydrogen peroxide and 2.5 mL of 65% concentrated nitric acid into the digestion tank in turn, and then put it into the microwave digestion instrument, using the se...

example 2

[0065] As described in Example 1, the smoking volunteer urine sample numbered B was selected, and the total amount of heavy metals in the 24-hour urine sample was measured as follows:

[0066] chromium nickel arsenic selenium cadmium HG lead µg µg µg µg µg µg µg 21.1443 8.8386 29.9560 21.9138 0.4376 0.2620 3.2630

example 3

[0068] As described in Example 1, the smoking volunteer urine sample numbered C was selected, and the total level of heavy metals in the 24-hour urine sample was measured as follows:

[0069] chromium nickel arsenic selenium cadmium HG lead µg µg µg µg µg µg µg 38.0987 2.4883 12.2719 15.0335 0.7927 0.7445 2.3468

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Abstract

The invention discloses a method for measuring the contents of chromium, nickel, arsenic, selenium, cadmium, mercury and lead in urine. The method comprises the steps as follows: collecting all urine of a smoker in 24 hours; storing in a polypropylene bucket at the temperature of subzero 80 DEG C; disinfecting the urine with microwaves; taking argon gas as carrier gas under acidic condition; measuring by using inductively coupled plasma mass spectrometry; and comparing with standard series and quantifying. The method has the advantages of accurate measurement result, good sensitivity and repeatability and the like, and is simple, convenient and quick to operate.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of urine collection, analysis and detection, and mainly relates to the technical field of determination of trace elements in urine, in particular to a method for accurately measuring chromium, nickel and arsenic in 24-hour urine of smokers by using inductively coupled plasma mass spectrometry , selenium, cadmium, mercury, lead content method. Background technique: [0003] As an important harmful element in cigarette smoke, heavy metals will enter the human body along with the mainstream smoke and cause certain harm to the human body. During the process of smoking cigarettes, the heavy metal elements in shredded tobacco can be dispersed in the mainstream smoke and sidestream smoke as smoke aerosols when the cigarette is burned, and will accumulate in the body after entering the body. Through the Fenton reaction, free radicals are generated, which will combine with DNA to cause oxidative damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/626
Inventor 侯宏卫田永峰张小涛刘彤陈欢韩书磊胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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