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Preparation method of collagen film for guided tissue regeneration

A technology to guide regeneration and collagen membranes, applied in medical science, surgery, etc., can solve the problems of loss of space maintenance ability, low tensile strength of collagen membranes, fast degradation speed, etc., and achieve excellent controllable degradation performance, excellent mechanical properties, good repair effect

Active Publication Date: 2013-05-01
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still some problems in the research of collagen film as GTR: first, the degradation rate in vivo is too fast, and second, compared with the requirements of GTR film, the tensile strength of collagen film is relatively small, and it is easy to collapse during application and lose the ability to maintain space

Method used

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  • Preparation method of collagen film for guided tissue regeneration
  • Preparation method of collagen film for guided tissue regeneration
  • Preparation method of collagen film for guided tissue regeneration

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0034] (1) Dissolve collagen in 0.1M acetic acid solution at 25°C to obtain a collagen solution with a concentration of 6 mg / ml;

[0035] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 7 with 0.1M sodium hydroxide solution, and keep it for 30 minutes;

[0036] (3) shear and stir the collagen solution obtained in step (2) with an electric mixer at 3000r / min for 5 minutes to obtain a collagen emulsion;

[0037] (4) Centrifuge the emulsion in step (3) at 2000r / min for 8 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0038] (5) Forming the collagen film obtained in step (4), then freeze-drying, and vacuum thermal crosslinking at 120°C;

[0039] (6) Packaging, irradiation sterilization.

[0040] Sample tear strength: 31.0MPa.

Embodiment 2

[0042] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0043] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 30°C to obtain a collagen solution with a concentration of 5 mg / ml;

[0044] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 5 with 0.1M sodium hydroxide solution, and keep it for 10 minutes;

[0045] (3) The collagen solution in step (2) is sheared and stirred for 5 seconds under the condition of 15000 r / min with a tissue masher to obtain a collagen emulsion;

[0046] (4) Centrifuge the emulsion in step (3) at 1000r / min for 10 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0047] (5) Forming the collagen film obtained in step (4), freeze-drying at -20°C, and thermally crosslinking under vacuum at 140°C;

[0048] (6) Soak the collagen membrane obtained in step (5) in 1% glutaraldehyde solution fo...

Embodiment 3

[0052] A method for preparing a collagen membrane for guided tissue regeneration, comprising the steps of:

[0053] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 4°C to obtain a collagen solution with a concentration of 10mg / ml;

[0054] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 8 with 0.1M ammonia water, and keep it for 10 minutes;

[0055] (3) The collagen solution obtained in step (2) is sheared and stirred for 15 seconds under the condition of 15000 r / min with a tissue masher to obtain a collagen emulsion;

[0056] (4) Centrifuge the emulsion in step (3) at 6000r / min for 5 minutes. At this time, the collagen floats on the surface of the solvent, and collect the collagen film on the solution;

[0057] (5) Forming the collagen film obtained in step (4), freeze-drying at -20°C, and vacuum thermal crosslinking at 110°C;

[0058] (6) Put the collagen film obtained in step (5) into 1% EDC-NHS solution (weigh a certain amount ac...

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Abstract

The invention discloses a preparation method of a collagen film for guided tissue regeneration. The method comprises the following steps of dissolving collagen in an acid solution, adjusting a pH (potential of hydrogen) value of an obtained solution to 5-9, continuing to conduct rapid shearing and stirring on the obtained solution, obtaining a collagen emulsion, centrifuging the obtained collagen emulsion, collecting the collagen film on the solution, allowing the obtained collagen film to be subjected to mold forming, freeze-drying, using a physical or / and chemical method for crosslinking, and obtaining the collagen film for the guided tissue regeneration. The obtained collagen film has excellent mechanical property and controlled degradation property, has a gradient pore structure, is suitable for transmission of nutrient substances and adsorption and multiplication of cells, and can better guide repair of defect tissues; the preparation technology is simple; the cost is lower; and the large-scale production is facilitated.

Description

technical field [0001] The invention relates to a preparation method of a collagen film for guiding tissue regeneration. Background technique [0002] Periodontal guided tissue regeneration (guided tissue regeneration, GTR) is currently the most advanced technology in the treatment of periodontal disease in the world. In 1982, Nymans et al. first proposed the concept of GTR (Nyman S.Lindhe J.Karring T, et al. Clin Periodontol.1982; 9(4):290-296). The core of GTR technology is the tissue-guided regeneration membrane, that is, by guiding The membrane acts as a space-isolated barrier membrane, while preventing gingival connective tissue cells and junctional epithelial cells from migrating to the root, while selectively allowing periodontal ligament cells and alveolar bone cells to adhere to the root surface and differentiate to form new periodontal attachments. This approach has been shown to be more effective in achieving clinical attachment and reducing probing depth than op...

Claims

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Application Information

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IPC IPC(8): A61L31/04
Inventor 任力季培红秦晓杰王迎军刘卅
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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