Method for identifying plasticizer in distilled spirit by caenorhabditis elegans
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A Caenorhabditis elegans and plasticizer technology, which is applied in the direction of material inspection products, animal husbandry, biological testing, etc., can solve the problems of complex operation, high cost, and high dependence on instruments, and achieve convenient experimental operation, low cost, Easy-to-grasp effects
Inactive Publication Date: 2013-05-01
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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[0005] The purpose of the present invention is to provide a method for identifying plasticizers in liquor by using Caenorhabditis elegans in or
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specific Embodiment approach 1
[0016] Specific embodiment one: the present embodiment utilizes Caenorhabditis elegans to identify the method for the plasticizer in white wine as follows:
[0017] 1. Weigh 1.0 g of cholesterol, dissolve it in absolute ethanol, and prepare a cholesterol ethanol solution with a concentration of 10 mg / ml;
[0018] 2. Preparation of phosphate buffer: weigh 13.6g KH 2 PO 4 , 1.8g KOH, add distilled water to 100mL, adjust the pH value to 6.0;
[0019] 3. Mix 15-20g agar powder, 2-3g peptone, 0.10-0.12g CaCl 2 , 0.11~0.13g MgSO 4 Add 2~3g NaCl and 2~3g NaCl into a 1000mL beaker, add 1ml cholesterol ethanol solution and 25mL phosphate buffer, and distill the volume to 1000ml with distilled water;
[0020] 4. Seal the beaker, put it in a high-pressure steam sterilizer at 120°C for 15-25 minutes, then cool it down to 50-60°C, add 0.5-5ml of white wine to be tested under a sterile environment, and then put the mixture in a sterile Pour it into a 6cm petri dish under the environmen...
specific Embodiment approach 2
[0022] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in step two, 16~19g agar powder, 2g peptone, 0.10g CaCl 2 , 0.11g MgSO 4 and 2g NaCl were added to a 1000mL beaker. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0023] Specific embodiment three: what this embodiment is different from specific embodiment one is that in step 2, 15g agar powder, 2g peptone, 0.10g CaCl 2 , 0.11g MgSO 4 and 2g NaCl were added to a 1000mL beaker. Others are the same as in the first embodiment.
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Abstract
The invention discloses a method for identifying a plasticizer in a stilled spirit by caenorhabditis elegans, and relates to a method for indentifying the plasticizer. The method solves the problems in a conventional plasticizer detection method that the operation is complex, the dependence degree for a device is large, and the cost is high. The method comprises the steps as follows: step one, preparing a cholesterol ethanol solution; step two, preparing a phosphate buffer solution; step three, preparing culture media of elegans and step four, coating the culture media of the elegans with a colon bacillus OP50 bacterium solution uniformly, culturing the culture media for 8-12h at the temperature of 37 DEG C, putting in 8-12 sexually mature caenorhabditis elegans, culturing for 12h, and then observing the egg-laying quantity of the elegans. If the egg-laying quantity of the elegans is lower than a normal level, the fact that a distilled spirit to be detected contains the plasticizer is indicated. The detection method has low requirements for experimental conditions, and does not require any special high-precision analytical instruments or detection devices, the experiment can be operated conveniently, the cost is low, and the method can be mastered easily.
Description
technical field [0001] The invention relates to a method for identifying plasticizers. Background technique [0002] Plasticizer, also known as plasticizer, is a phthalate homologue. Since May 2011, six phthalate plasticizers including DEHP, DINP, DNOP, DBP, DMP, and DEP have been detected in food in Taiwan, and DIDP has been detected in medicines. On June 1, the Ministry of Health issued an emergency release The announcement lists phthalates (also called phthalates) as non-edible substances that may be illegally added to food and food additives that are easily abused. However, at the end of 2012, it was exposed that the plasticizer of "drunkard" wine exceeded the standard by 2.6 times, which caused people's panic and doubts about the plasticizer and the food safety of liquor, especially high-end wine. [0003] Plasticizer is an environmental hormone that can cause immunity and reproductive decline in humans and animals. At present, the tolerable daily intake of 60 kg adu...
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IPC IPC(8): G01N33/50A01K67/033
Inventor 王云彪
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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