Immunologic adjuvant for preventing toxoplasma infection and application of immunologic adjuvant
A technology of infection prevention and immune adjuvant, which is applied in the direction of anti-infective drugs, medical preparations containing active ingredients, bacterial antigen components, etc., and can solve problems such as unsatisfactory recombinant vaccines
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[0082] The immune adjuvant used for the prevention of Toxoplasma gondii infection, which uses pVAX plasmid as a carrier and is a recombinant plasmid pVAX-IL21-IL15 fused with interleukins IL21 and IL15, its base sequence is shown in SEQ ID NO: 1.
[0083] The construction method of pVAX-IL21-IL15 recombinant plasmid is as follows:
[0084] 1. Extraction of total RNA from the spleen of KM mice: Seventy-two hours after intraperitoneal inoculation of the RH strain of Toxoplasma gondii, the KM mice were killed by cervical dislocation, soaked in 75% ethanol solution for 2-3 minutes, and the spleen was removed aseptically. Put the spleen into an RNase-Free centrifuge tube, and follow-up experiments were performed according to the instructions of the RNAprep Pure Tissue Kit kit from Tiangen Biochemical Technology (Beijing) Co., Ltd.;
[0085] 2. RT-PCR amplification of the target fragment: RT-PCR amplification of IL21 and IL15 gene sequences was performed using the total RNA of t...
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