Bubble eliminating device for high-throughput microfluidics cell chip and operation method thereof

Abstract

The invention relates to the technical field of high-throughput microfluidics cell chips and discloses a bubble eliminating device for a chip and an operation method thereof. Tip heads filled with liquid are respectively connected at the inlet and the outlet of the chip. The bubble eliminating device comprises a base and a seat body, wherein a groove for placement of the chip is arranged at the upper end of the base, the groove is sealed by the seat body, two concave cavities are arranged at the lower end of the seat body, the upper end of the seat body is sealed, and two through holes which are respectively and correspondingly communicated with the two concave cavities and are used for inletting the external inert gases into the concave cavities are arranged on side walls of the base. The bubble eliminating device is not designed for certain chips and has strong applicability, the chip only needs to be placed in the groove of the base, and the bubble eliminating device is simple to manufacture and has a simple structure, achieves bubble eliminating effect by utilizing positive pressure, has high bubble eliminating efficiency, does not affect and interrupt liquid flow entering the microfluidics cell chip, guarantees the normal state and function of the growth of the cell, is simple to operate, does not interrupt the liquid flow, guarantees the normal operation of the experiment, and does not have potential affect on the experiment.

Description

technical field [0001] The invention relates to the technical field of high-throughput microfluidic cell chips, in particular to a debubbling device for high-throughput microfluidic cell chips and an operating method thereof. Background technique [0002] Microfluidic chip technology can integrate thousands of screening units with a volume of only nanoliters or picoliters on a small area. , the great potential of low-cost ultra-high-throughput drug screening technology. The throughput of most of the microfluidic cell chips reported so far is still very low, and has not even exceeded the throughput commonly used in microwell plate technology. There are many challenges, including technical challenges such as uniform distribution of cells in microculture unit arrays, suspension cell culture, and solution delivery. [0003] At present, in response to the above challenges, the positive photoresist and negative photoresist are combined with overlay to produce the microfluidic ch...

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a degassing device for high-throughput microfluidic cell chips, aiming to solve the problem of poor applicability, complex structure and complicated manufacture of the degassing device for high-throughput microfluidic cell chips in the prior art and issues with potential adverse effects on the experiment

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