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Method for staining microorganisms

A dyeing method and microbial technology, applied in the preparation of test samples, etc., to achieve high water solubility, obvious dyeing effect, and low cost

Inactive Publication Date: 2015-04-29
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of food coloring for microbial staining

Method used

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  • Method for staining microorganisms
  • Method for staining microorganisms
  • Method for staining microorganisms

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, utilize food coloring to carry out staining to escherichia coli

[0033] 1. Preparation of pigment medium

[0034] The pigment medium is prepared from the following raw materials per liter: tryptone 10g, yeast extract 5g, NaCl 5g, pigment, add water to make up to 1L. The pH=7 of the pigment medium. The preparation method is as follows: take 10 g of tryptone, 5 g of yeast extract, 5 g of NaCl, and 5 g of pigment, add water to make the volume to 1 liter, and sterilize the culture based on damp heat at 121° C. for half an hour. The pigments are sunset yellow, erythrosine, carmine, fruit green or scarlet.

[0035] The pigment medium whose pigment is sunset yellow is called sunset yellow medium, and the content of sunset yellow in the sunset yellow medium is 5g / L.

[0036] The pigment medium whose pigment is erythrosine is called erythrosine medium, and in the erythrosine medium, the content of erythrosine is 1 g / L.

[0037] The pigment medium whose pigment...

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Abstract

The invention discloses a method for staining microorganisms. The method comprises the following steps of: culturing the microorganisms to be stained in a pigment culture medium, and carrying out vital cell staining on the microorganisms to be stained, wherein the pigment culture medium is composed of an edible pigment and a culture medium for culturing the microorganisms to be stained; and the concentration of the edible pigment in the pigment culture medium is 0.5g / L to 20g / L. The method for staining the microorganisms, disclosed by the invention, is simple to operate, remarkable in staining effect, low in cost and harmless to thalluses because of nontoxic staining.

Description

technical field [0001] The invention relates to a method for staining microorganisms. Background technique [0002] At present, there are many staining methods for bacteria, and the staining of bacteria is divided into living bacteria staining and dead bacteria staining. Bacterial staining methods commonly used in microbiology include Gram staining, acid-fast staining, spore staining, and capsule staining. Among them, Gram staining: the decolorization step in the traditional four-step Gram staining method is not easy for beginners to master. Excessive decolorization will stain gram-positive bacteria red, and insufficient decolorization will stain gram-negative bacteria. positive bacteria. Acid-resistant dyeing: The traditional method is to add carbolic acid to the bacterial film first and then warm it up. The dye solution is easy to dry up in this method, and dyes need to be added continuously, and the amount of dyes is relatively large. Spore staining: The commonly used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 凌焱陈惠鹏曲芬李玉霞吴逊李炳娟白东梅李伟东刘刚周围李北平高原岳俊杰梁龙
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE