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Method for visual rapid detection of clenbuterol by adopting nanogold probe

A nano-gold probe and clenbuterol technology are applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of time-consuming, expensive, and low sensitivity. , to achieve the effect of simple determination method, rapid detection and low cost

Inactive Publication Date: 2013-05-08
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods can realize the detection of clenbuterol, but there are disadvantages such as high false positive rate, cumbersome process, time-consuming, low sensitivity, expensive instruments and high cost.

Method used

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  • Method for visual rapid detection of clenbuterol by adopting nanogold probe
  • Method for visual rapid detection of clenbuterol by adopting nanogold probe

Examples

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Effect test

Embodiment 1

[0018] Sodium acetate and acetic acid were used to prepare the buffer solution, and ultrapure water was used to prepare solutions such as gold chloride, sodium citrate, melamine, clenbuterol hydrochloride, ractopamine, and albuterol. The solvent used was 0.01M acetic acid-sodium acetate buffer solution with pH 5.0, and the concentrations of chloroauric acid, sodium citrate and melamine were 1×10 -3 M, 38.8×10 -3 M and 3.0×10 -7 M. Sodium citrate is added to the gold chloride solution for reduction to generate nano-gold, and the color of the solution is wine red at this time.

Embodiment 2

[0020] Add acetic acid-sodium acetate buffer solution and melamine solution of pH 5.0 to the synthesized gold nanometer solution, and stir for 15 minutes to prepare a melamine-modified gold nanometer probe solution. At this time, the color of the solution is still wine red.

Embodiment 3

[0022] After adding clenbuterol hydrochloride, ractopamine, and albuterol to the synthesized gold nanoprobe solution, the gold nanoprobes changed from a dispersed state to an aggregated state, and at the same time, the color of the solution changed from wine red to purple, and the corresponding UV absorption The spectrum also changes, and clenbuterol, ractopamine, and albuterol can be quantified by the naked eye or by a spectrophotometer.

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Abstract

The invention relates to a method for visual rapid detection of clenbuterol by adopting a nanogold probe. According to the method, contents of three main components, namely clenbuterol hydrochloride, ractopamine and salbutamol, are visually detected by specifically using melamine-modified nanogold. The method is mainly characterized in that the nanogold probe is formed by modifying melamine onto the surface of nanogold. The nanogold probe can specifically react with the clenbuterol hydrochloride, the ractopamine and the salbutamol through hydrogen bonds to cause polymerization of the nanogold and color change of a solution. According to a color change scope of the solution, whether the clenbuterol hydrochloride, the ractopamine and the salbutamol exist in the solution and the contents of the clenbuterol hydrochloride, the ractopamine and the salbutamol can be judged with the naked eye. The detection method is simple and practicable, is low in cost and high in sensitivity and is suitable for spot rapid detection of batch samples.

Description

technical field [0001] The present invention relates to a method for rapidly detecting clenbuterol based on the visualization of nano-gold probes, specifically refers to the visual detection of three main components of clenbuterol, clenbuterol hydrochloride, ractopamine and albuterol. Background technique [0002] The issue of food safety has drawn more and more attention from the society. In recent years, clenbuterol has become the key word of food safety. Clenbuterol, ractopamine, and albuterol are commonly found in clenbuterol. Clenbuterol, as a vegetative neuromodulator, can reduce Mg in the blood that inhibits muscle excitability 2+ Concentration, indirectly promote muscle excitement, inhibit protein decomposition and fat synthesis, and then increase protein production. However, clenbuterol remains in livestock and poultry for a long time, and humans are particularly sensitive to β-adrenergic agonists. Because clenbuterol can accelerate the decomposition of fat, so ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 赵红周影苏晓鸥王培龙何裕建袁龙飞蔡波太刘庆菊
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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