Method for isolating and culturing human meningioma cells

Abstract

The invention belongs to the biomedical field and relates to a method for isolating and culturing meningioma cells and in particular relates to a method for isolating meningioma cells from isolated human meningioma tissues and subculturing the meningioma cells. The method is characterized by adopting a cocktail type combined enzyme to digest the isolated human meningioma tissues stepwise to obtain meningioma cell suspension and carrying out low-oxygen culture on the meningioma cell suspension in a specific culture solution to obtain primary meningioma cells; and carrying out suspension culture on the obtained primary meningioma cells in the specific culture solution under the low-oxygen condition until more than 15 generations of meningioma cells are obtained. The method has the advantages that the cocktail type combined enzyme and the stepwise digestion method are beneficial to preserving cell viability, so that the cells obtained through digestion are basically viable cells, thus providing cell sources for primary culture of meningioma cells; and the method is beneficial to isolation and proliferation of meningioma cells and provides cell sources for further carrying out meningioma mechanism and drug screening researches.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for cell separation and culture, in particular to a method for the separation and culture of meningioma cells, in particular to a method for separating and culturing meningioma cells from isolated human meningioma tissues. Background technique [0002] According to reports, meningioma is a common intracranial tumor, accounting for about 20% of the total number of intracranial tumors, and the incidence rate is higher in women. Studies have shown that most meningiomas are benign, with a long course of disease, and their distribution is roughly similar to that of arachnoid granules, usually in the parasagittal sinus of the cerebral hemisphere. Surgical resection is the preferred clinical treatment method. Treatment practices show that some meningiomas have a higher recurrence rate 5 years after resection; and some tumors are difficult to achieve total surgical resection because of t...

AI Technical Summary

Problems solved by technology

[0003] At present, the factors that make it difficult to obtain a large number of primary and subcultured meningioma cells are as follows: (1) The interstitial cells of meningioma tissue include many stroma / fibers, and it is difficult to digest meningioma tissue by mechanical separation or single enzyme method , hard mechanical separation or enzyme digestion for a long time, resulting in cell fragmentation, so a large number of living cells cannot be obtained from the tissue; (2) Since most meningiomas are benign, the cells grow slowly and cannot crawl to the cell-free cells far away from the tissue block Zone growth, can only climb out and grow around the tissue block

Therefore, it is difficult for the cells to cover the bottom of the culture bottle; (3) due to the slow growth rate of the cells, when the monolayer cells are adhered to the wall for culture, it is difficult to pass on for a long time with conventional cell seeding density; (4) a suitable method has not been established. The culture system of meningioma cells, the growth factors or hormones that can promote the proliferation of meningioma cells are not clear

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