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Sodium cyclamate detection method

A detection method and cyclamate technology are applied in the detection field of food additives, which can solve the problems of time-consuming, cumbersome operation steps, unfavorable rapid analysis and determination, etc., and achieve the effects of low cost and simple operation.

Inactive Publication Date: 2013-05-15
QINGDAO HARRENS INSPECTION & TESTING INC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the detection of cyclamate in food, the latest national standard (GB5009.97-2003) adopts gas chromatography, and there are also literature reports on colorimetry, thin layer chromatography, etc. The main disadvantages of these methods are that the operation steps are too cumbersome, time-consuming, Facilitate rapid analysis and determination

Method used

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  • Sodium cyclamate detection method

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1 The cyclamate detection method of the present embodiment comprises the following steps:

[0018] (1) Use sodium hypochlorite to derivatize the sodium cyclamate standard

[0019] Measure 1mL, 0.6mL, 0.4mL, 0.2mL, 0.05mL of 500mg / L standard working solution of cyclamate (respectively corresponding to the final concentration: 100mg / L, 60mg / L, 40mg / L, 20mg / L, 5mg / L L) In a 50ml centrifuge tube, add 20ml of water, 5ml of n-hexane, 1:1 sulfuric acid solution (concentrated sulfuric acid and water volume ratio 1:1) 2ml, 1:2 sodium hypochlorite solution (sodium hypochlorite and water volume ratio 1:2) 1ml, Vortex for 3 minutes; transfer all the n-hexane layer to a 10ml centrifuge tube, add 10ml of 10% sodium bicarbonate solution, vortex for 3 minutes, pass the n-hexane layer through a 0.45 μm organic phase filter, and set aside;

[0020] (2) Adopt liquid chromatography to be equipped with ultraviolet detector to detect the cyclamate standard substance after derivat...

Embodiment 2

[0027] Embodiment 2 The detection steps of the standard recovery of the inventive method in this embodiment are as follows:

[0028] Using sample spiked recovery rate test, add 0.05ml, 0.25ml, 0.5ml of standard solution with a concentration of 500mg / L to the sample, corresponding to the spiked amount of 25μg, 125μg, 250μg respectively; take the sample without standard solution as the blank control Each sample is detected 3 times respectively, and detection method is with embodiment 1, and response value spectrogram sees respectively Figure 8 , Figure 9 , Figure 10 , Figure 11 Calculate the recovery of standard addition of method cyclamate of the present invention according to analysis result, as shown in table 1:

[0029] Table 1 Statistical table of recovery rate of cyclamate

[0030]

[0031] As can be seen from Table 1, the recovery rate of the cyclamate detection method of the present invention is high and the repeatability is good. To sum up, the method for de...

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Abstract

The invention relates to a food additive detection method and in particular relates to a sodium cyclamate detection method in drink food. The sodium cyclamate detection method is characterized by comprising the following steps: (1) performing derivatization on a sodium cyclamate standard substance by using sodium hypochlorite; (2) detecting the sodium cyclamate standard substance subjected to the derivatization by employing a liquid-chromatogram-matched ultraviolet detector; (3) drawing a sodium cyclamate standard curve according to the detection result in the step (2); (4) performing the derivatization on a sample to be detected; and (5) detecting the sodium cyclamate in the sample to be detected by employing the method in the step (2), and calculating the sodium cyclamate content in the sample to be detected according to the standard curve. The sodium cyclamate detection method is easy to operate, sensitive, rapid, low in cost and high in repeatability.

Description

technical field [0001] The invention relates to a detection method of food additives, in particular to a detection method of cyclamate in beverages and foods. Background technique [0002] In recent years, food safety has become a public health concern, and the abuse of additives has become an important factor affecting food safety. The domestic market survey found that the excessive use of additives in food in our country is more serious. Cyclamate, whose chemical name is sodium cyclamate, is a commonly used additive in food production. Cyclamate is a commonly used sweetener, and its sweetness is 30 to 40 times that of sucrose. If consumers often consume beverages or other foods with excessive cyclamate content, they will consume too much, which will cause harm to the human liver and nervous system, especially for the elderly, pregnant women and children with weak metabolic detoxification ability. Cyclamate is a widely consumed artificial sweetener in the United States, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 傅鹏刘刚
Owner QINGDAO HARRENS INSPECTION & TESTING INC
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