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Bispecific monoclonal antibody resisting clenbuterol hydrochloride and olaquindox and preparation method of bispecific monoclonal antibody

A clenbuterol hydrochloride, monoclonal antibody technology, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc. stable effect

Inactive Publication Date: 2013-05-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0012] At present, although there are reports on immunoassay techniques for clenbuterol hydrochloride and olaquindox, they are all limited to a single analysis technique and method. In the context of the need for multi-residue analysis, it is necessary to establish a method that can simultaneously detect two or two types of target compounds. Immunoassay technology is particularly urgent, and the preparation of high-quality bispecific monoclonal antibodies is a new means and method for establishing immunological multi-residue analysis technology

Method used

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Examples

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the examples.

[0024] 1. Preparation of Immunogen

[0025] 1.1 Preparation of clenbuterol artificial antigen

[0026] 1.1.1 Activation of clenbuterol hydrochloride

[0027] Take 3 mg CL, add 1 mL 1mol / L HCL and 500 μL distilled water into the reaction beaker, adjust the pH to 0.5 at the same time, then add 60 μL 0.2 mol / L NaNO continuously and slowly at 4 °C 2 , 4 ° C low-speed stirring for 1-3 h.

[0028] 1.1.2 Synthesis and purification of artificial antigen

[0029] Accurately weigh bovine serum albumin (BSA) or ovalbumin (OVA) and dissolve in phosphate buffered saline (PBS) with a pH value of 7.0-8.0. Slowly drop the clenbuterol hydrochloride after azo into the bovine serum albumin solution (the molecular ratio of clenbuterol hydrochloride and protein carrier is between 20-30), adjust the pH with 1 mol / L sodium hydroxide at Between 7.0-8.0, continue to stir in the dark for 2-5 h after th...

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Abstract

The invention discloses a bispecific monoclonal antibody resisting clenbuterol hydrochloride and olaquindox and a preparation method of the bispecific monoclonal antibody, belonging to the field of biotechnology. The bispecific monoclonal antibody has specificity to both the clenbuterol hydrochloride and the olaquindox. The bispecific monoclonal antibody acquired by the preparation method disclosed by the invention has the advantages of stable properties and high detection sensitivity, can be abundantly produced and provides the technological base for fast detection of the clenbuterol hydrochloride and the olaquindox.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bispecific monoclonal antibody against clenbuterol hydrochloride and olaquindox and a preparation method thereof. Background technique [0002] Immunoassay (IA) is a technology that uses antibodies as biochemical detectors to perform qualitative and quantitative analysis of compounds, enzymes, or proteins. It is based on the specific and reversible binding reaction between antigens and antibodies. It has the characteristics of strong specificity, high sensitivity, convenience and speed, high throughput, low detection cost, safety and reliability. This method generally does not require expensive instruments, and the technical requirements of the users are not high. It is easy to popularize and promote, and is especially suitable for on-site screening and rapid analysis of a large number of samples. A series of detection products developed based on this technology, such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/44C12N5/20C12N5/18C12R1/91
Inventor 金仁耀
Owner ZHEJIANG GONGSHANG UNIVERSITY
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