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Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition

A microbial composition, the technology of acetochlor, applied in the directions of microorganisms, microorganisms, microorganism-based methods, etc., can solve the problem of low degradation ability of acetochlor

Inactive Publication Date: 2013-05-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, researchers have isolated some microorganisms that can degrade acetochlor, but generally the ability to degrade acetochlor is not high: Xu et al. reported the degradation of acetochlor by Pseudomonas oleovorans LCa2, and the strain could degrade 7.6 mg of acetochlor within 7 days ·L -1 98.03% of acetochlor degradation

Method used

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  • Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition
  • Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition
  • Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Screening and isolation of acetochlor-degrading strain Rhodococcus sp.T3-1

[0045] Take 5g soil sample and place in 100mL containing 25mg·L -1 Acetochlor-enriched medium (g L -1 : NH 4 NO 3 1.0, KH 2 PO 4 0.5,K 2 HPO 4 1.5, NaCl1.0, MgSO 4 ·7H 2 O0.1, yeast juice 0.02, pH7.0. ), at 30°C, 180r·min -1 Culture 5d. The degradation of acetochlor by the enrichment solution was measured with a UV scanner, and it was found that after acetochlor was degraded, it was inserted into 50mg·L with a 10% inoculation amount -1 In the acetochlor enriched medium, continue to enrich and measure the degradation, follow this method until the concentration of acetochlor increases to 100mg·L -1 , and passaged 3 times.

[0046] The acetochlor-enriched solution was serially diluted and then spread on LB plates and mixed with 100 mg·L -1 MSM plates with acetochlor as the only carbon source (inorganic salt medium, g L -1 : NH 4 NO 3 1.0, KH 2 PO 4 0.5,K 2 HPO 4 1.5...

Embodiment 2

[0050] Example 2: Screening and isolation of 2'-methyl-6'-ethyl-2-chloroacetanilide-degrading strain Delftia sp.T3-6

[0051] The different forms of single colonies obtained by streaking in Example 1 were purified and inoculated in 3mL liquid LB medium for culture, the bacteria were collected by centrifugation, and inoculated in the culture medium containing 25mg / L 2'-methyl-6'-ethyl-2 In the inorganic salt medium of -chloroacetanilide, at 30°C, 180r·min -1 After incubating on a shaking table for 2 days, add an equal volume of dichloromethane to the sample to be determined, vibrate vigorously for 1 min, let it stand for stratification, suck out the water phase, add enough anhydrous sodium sulfate to remove the residual water in the dichloromethane, and Ultraviolet scanning is carried out in the wavelength range of 200-350nm, and the maximum absorption peak of 2'-methyl-6'-ethyl-2-chloroacetanilide is 228nm. Degradability of 6'-ethyl-2-chloroacetanilide. Through ultraviolet s...

Embodiment 3

[0054] Example 3: Screening and isolation of 2-methyl-6-ethylaniline degrading strain Sphingobium sp.MEA3-1

[0055] With the single colonies of different forms obtained by streaking in Example 1, after purification, they were inoculated in 3mL liquid LB medium and cultivated for 24h, and were inoculated with 5% inoculum in the culture medium containing 50mg / L2-methyl-6-ethylaniline ( MEA) inorganic salt medium (g L -1 : NH 4 NO 3 1.0, KH 2 PO 4 0.5,K 2 HPO 4 1.5, NaCl1.0, MgSO 4 ·7H 2 O0.1, pH7.0. ), at 30°C, 180r·min -1 After 2 days of incubation on a shaking table, extract with dichloromethane, and detect the degradation of MEA by ultraviolet scanning. -1 The MEA is completely degraded ( Figure 8 ), through identification, the strain belongs to the genus Sphingobium. Named Sphingobium sp.MEA3-1. The accession number of 16SrRNA in Genbank is JN595860.

[0056] Inoculate a single colony of Sphingobium sp.MEA3-1 in 3mL liquid LB medium, 30°C, 180r min -1 Cultivate...

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Abstract

The invention belongs to the technical field of biological treatment and discloses a microorganism composition for degrading acetochlor and / or butachlor and an application of the microorganism composition. The microorganism composition comprises Rhodococcus sp. T3-1, Delftia sp. T3-6 and / or Sphingobium sp. MEA3-1 which are all preserved in China Center for Type Culture Collection on December 15, 2012, wherein preservation numbers of the microorganism strains are respectively CCTCC NO: M2012525, CCTCC NO: M2012526 and CCTCC NO: M2015527. The microorganism composition can be applied in combined degradation of acetochlor and / or butachlor.

Description

technical field [0001] The invention belongs to the technical field of biological treatment, and relates to a microbial composition for degrading acetochlor and / or butachlor and an application thereof. Background technique [0002] Chloroacetamide herbicides are a kind of high-efficiency and highly selective contact herbicides, which have a very significant killing effect on grass weeds. The main representative varieties of acetochlor and butachlor are the most used in my country For two of the three herbicides, the annual usage exceeds 10,000 tons and 5,000 tons, respectively. Chloroacetamide herbicides are highly toxic to fish. Acetochlor and butachlor are classified as B-2 carcinogens by the U.S. Environmental Protection Agency. It is a huge threat to the ecological environment and human health. In addition, chloroacetamide herbicides have hidden phytotoxicity to crops, causing serious losses to agriculture. [0003] Biodegradation is an important transformation process...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/04A62D101/26A62D101/28C02F101/38C02F101/34
Inventor 崔中利侯颖王飞黄艳
Owner NANJING AGRICULTURAL UNIVERSITY
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