Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof
A technology of photosynthetic bacteria and corncobs, applied in chemical instruments and methods, fixed on/in organic carriers, biological water/sewage treatment, etc., can solve the difficulty of solid-liquid separation, reduce lake nutrients, and time-consuming spraying, etc. question
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[0029] A method for preparing corn cobs and / or stalks loaded with photosynthetic bacteria, comprising the following steps:
[0030] 1) Clean the corn cob and / or straw, then put it in a closed high-temperature sterilization tank, and sterilize it for 1-3 hours under the conditions of a pressure of 0.1-0.5MPa and a temperature of 110°C to obtain sterilized corn cobs and / or straw;
[0031] 2) Put the sterilized corncobs and / or straws obtained in step 1) into the mixed solution for the first soaking for 1-12 hours, and then air-dry for 1-5 hours to obtain the air-dried corncobs and / or straw,
[0032] Wherein, the mixed solution includes raw materials with the following mass percentage concentrations: 0.1-1% chitosan; 1-5% sodium alginate; 1-5% K 2 HPO 4 ;
[0033] 3) Soak the air-dried corncobs and / or stalks in a 2.0×10 9 ~3.1×10 10 cfu / mL of photosynthetic bacteria strain solution for 1 to 6 hours, then take it out, and store it in a sealed environment at -5 to 20°C or di...
Embodiment 1
[0038] Wash and dry the corncobs, place them in a 0.2MPa high-temperature sterilization tank and seal them for 2 hours; take out and soak them in chitosan with a mass percentage concentration of 0.5%, 0.5% sodium alginate and 3% K 2 HPO 4 in the mixed solution, soaked for 12 hours, took it out and air-dried for 5 hours, and then soaked in 2.0×10 9 ~3.1×10 10cfu / mL of photosynthetic bacteria solution for 6 hours; then string them together with ropes and fix them on driftwood, put them in eutrophic lakes and absorb the phosphorus and nitrogen in the water, causing the cyanobacteria to lose nutrients and die, so as to control polluted lakes the goal of. After 3 days, the dead cyanobacteria adsorbed on its surface were shaken off, and when the algaecidal effect of the corncob loaded with photosynthetic bacteria decreased, the step of loading photosynthetic bacteria could be carried out again to regenerate them.
[0039] Get 1 corn cob attached with photosynthetic bacteria obtai...
Embodiment 2
[0043] Cut the corn stalks, each corn stalk has a length of 40-50 cm after cutting, punch holes with a mace, wash and dry, and place in a 0.5 MPa high-temperature sterilization tank for sealing treatment for 3 hours. Take out the chitosan that is soaked in mass percent concentration and be 0.5%, the sodium alginate of 0.5% and the K of 3% 2 HPO 4 in the mixed solution. Soak for 8 hours, take it out and air-dry for 3 hours, then soak in photosynthetic bacteria (the number of bacteria is 2.0×10 9 ~3.1×10 10 cfu / mL) solution for 1 hour; then string them together with ropes, fix them on driftwood, and put them in eutrophic lakes to kill blue-green algae and control polluted lakes. After 10 days, shake off its surface to adsorb dead blue-green algae. When the algicidal effect of the corn stalk loaded with photosynthetic bacteria decreases, the step of loading photosynthetic bacteria can be carried out again to make it regenerate.
[0044] Get 50 corn stalks loaded with photosy...
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