Aflatoxin M1 detection kit

Abstract

The invention relates to an aflatoxin M1 detection kit, and belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), wherein the aflatoxin M1 detection kit is used for detecting aflatoxin M1 (short for AFM1) content in milk products. The aflatoxin M1 detection kit comprises a box body, a 24-cell AFM1 coated reaction plate inside the box, a reaction cover plate, 12 bottles of reagents, concave bottle positions for placing the reagents, an ice bag, a frame, an instruction manual, a packet of water absorption paper, two disposable droppers and a quality detection report. The aflatoxin M1 detection kit is characterized in that the coated reaction plate adopts a 24-well reagent plate as a solid phase carrier, and the 12 bottles of the reagents comprise 6 bottles of AFM1 standard substance solutions, enzyme labeled antigen, an enzyme labeled antigen dilution buffer solution, a concentration washing solution, a substrate solution, a coloration solution and a termination solution, and the number of the concave bottle positions is 16. The kit has characteristics of simple structure, easy use, low price, and high sensitivity, wherein the sensitivity can be more than 0.l ng / ml.

Description

technical field [0001] A method for detecting aflatoxin M 1 The test kit belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), and is used for the detection of aflatoxin M in dairy products. 1 (referred to as AFM 1 ) content detection. Background technique [0002] Aflatoxin (AFM 1 ) is a group of strong secondary metabolites produced by the fungus Aspergillus flavus and A. parasilicus strains. More than 20 kinds of aflatoxins (AFT) have been found, of which aflatoxin B1 is toxic and carcinogenic strongest substance. Aflatoxin M1 is a hydroxylated metabolite of aflatoxin B1 and is also a strong carcinogen. When mammals ingest AFB 1 After contaminated feed, AFM will be converted to AFM by hydroxylation in vivo 1 secreted in milk. Dairy cows ingested aflatoxin B 1 Contaminated feed contains aflatoxin M in milk 1 . Due to aflatoxin M 1 It is quite stable and cannot be killed by pasteurization, so the detection of aflatoxin M 1 Not only animal...

AI Technical Summary

Problems solved by technology

The former two have the disadvantages of cumbersome sample pretreatment steps, time-consuming and laborious, expensive instruments, and the need for professional operation, etc., while immunological analysis has strong specificity, simple sample pretreatment, low cost, and is harmful to experimenters and the environment. Pollution hazard is small and other advantages

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