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Cultivation method of chromosomal-translocation triploid seedless watermelons

A chromosomal translocation, seedless watermelon technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of no commercial value, cumbersome processing of female flowers, inability to grow seedless watermelons, etc. The effect of expanding the planting area

Inactive Publication Date: 2013-06-26
TIANJIN KERNEL VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because 3X seedless watermelon tends to appear colored seeds when the temperature difference between day and night is large, it directly affects the commerciality of seedless watermelon and restricts the development of the seedless watermelon industry, especially in watermelon eugenics areas (regions with large temperature difference between day and night) ) cannot grow seedless watermelons, which is also a breeding problem that is difficult to overcome in current triploid watermelon breeding; the second method is to utilize hormones to cultivate seedless watermelons.
This method is cumbersome to deal with female flowers, and the resulting seedless watermelon has a very high rate of colored seeds and has no commercial value. At present, it has not been used in watermelon production in various countries.

Method used

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  • Cultivation method of chromosomal-translocation triploid seedless watermelons
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  • Cultivation method of chromosomal-translocation triploid seedless watermelons

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The cultivation method of chromosomal translocation triploid seedless watermelon, comprises the steps:

[0023] A, the breeding of male parent: the diploid watermelon breeding material jubilee (abbreviation Jb) of chromosomal translocation is crossed with the diploid material smokylee (abbreviation Sm) that is suitable for making seedless watermelon male parent, adopts continuous backcross 4 generations ( Each backcross progeny selects a single melon with few seeds for backcrossing); self-crossing 1 generation, test crossing 1 generation (selecting a single melon with many seeds and common 2X for test crossing, and selecting a test cross for a single melon with few seeds), The male parent of chromosomal translocation triploid seedless watermelon 2X translocation (smokylee allelic line of chromosomal translocation) can be obtained, code-named SS-3; (see figure 1 )

[0024] B, the breeding of female parent: the mutant strain of Yu No. 6 in the diploid watermelon breeding...

Embodiment 2

[0027] Variety comparison test of "Jinmi No. 8"

[0028] In the spring of 2006-2007, a comparison test of seedless watermelon varieties was carried out in the greenhouse of Baodi District Biological Center. Heimi No. 2 was used as the control, arranged in random blocks, and three repetitions were set up. 20 plants were planted in each plot, with an area of ​​25m2. 2 , plant spacing 0.5m, row spacing 2.5m, single row planting, three vine pruning, each plant leaves the third female flower and one fruit sitting behind. Furrow planting with plastic film covering, sandy soil, medium fertility, 667m 2 Apply 500kg of chicken manure, 30kg of diammonium phosphate, 20kg of urea, raise seedlings on March 5, and transplant on April 7. The test results are shown in Table 1 for the high yield and Table 2 for the quality.

[0029] Table-1 Comparison results of high yield of Jinmi 8

[0030]

[0031]

[0032] Table-2 Quality comparison results of Jinmi No. 8

[0033]

[0034] See...

Embodiment 3

[0036] Test for Seedlessness of Chromosomal Translocation Triploid Watermelon

[0037] The SS-3 chromosomal translocation material was used as the male parent, and different types of 4X were used as the female parent to conduct a seedless test. The experiment was conducted in Tianjin and Shaanxi, and each combination was repeated 3 times, with 20 plants in each repetition. When the fruit was ripe, the coloring species of single melon were investigated. The test results are shown in Table-3.

[0038] Table-3 Chromosomal translocation triploid watermelon seedless test

[0039]

[0040] It can be seen from Table-3 that the triploid seedless watermelon cultivated by chromosomal translocation has almost no colored seeds. It shows that chromosomal translocation abortion and 3X abortion can be superimposed effectively.

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Abstract

The invention discloses a cultivation method of chromosomal-translocation triploid seedless watermelons. The cultivation method comprises the following steps of: (a) breeding of a male parent, namely crossing a chromosomal-translocation diploid watermelon breeding material with a diploid material suitable for serving as a seedless watermelon male parent, thus obtaining the male parent in a breeding method for four-generation continuous backcross, one-generation self cross and one-generation test cross; (b) breeding of a female parent, wherein mutating a diploid watermelon breeding material suitable for being mutated into tetraploid watermelons into tetraploid watermelons by a colchicine solution with the mass concentration of 0.2%, thus obtaining the female parent by self-crossing fertile restoration for 3-4 generations; and (c) performing cross matching on the female parent and the male parent to obtain the chromosomal-translocation triploid seedless watermelon variety through variety tests. The cultivation method disclosed by the invention has the advantages that chromosomal translocation and 3X double abortion are superposed, so that the breeding difficulty of more colored and abortive grains of 3X seedless watermelons is effectively overcome; and when the chromosomal-translocation triploid seedless watermelons are planted in protected areas and areas with large temperature difference at day and night, the colored and abortive grains do not occur, so that the planting range is expanded.

Description

technical field [0001] The invention belongs to plant breeding, in particular to a method for cultivating seedless watermelons. Background technique [0002] At present, there are two methods for cultivating seedless watermelon. The first method is to use 4X as the female parent and common 2X watermelon as the male parent to crossbreed and cultivate 3X seedless watermelon varieties, which are widely used in production at present. However, because 3X seedless watermelon tends to appear colored seeds when the temperature difference between day and night is large, it directly affects the commerciality of seedless watermelon and restricts the development of the seedless watermelon industry, especially in watermelon eugenics areas (regions with large temperature difference between day and night) ) can not grow seedless watermelons, which is also a breeding problem that is difficult to overcome in current triploid watermelon breeding; the second method is to utilize hormones to cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 焦定量张艳宁商纪鹏马运生董家行许向路高尚曾郗颖
Owner TIANJIN KERNEL VEGETABLE RES INST
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