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Proliferation culture medium for aristolochia longgonensis tissue culture and application of proliferation culture medium

A proliferation medium and tissue culture technology, applied in the fields of application, horticulture, botany equipment and methods, etc., can solve the problems of different species, large differences in growth conditions, and different distribution regions, and achieve the effect of improving the effect of proliferation

Inactive Publication Date: 2013-06-26
广西橡胶研究所
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the Aristolochia nongang and Aristolochia akebs involved in the present invention are distributed in different regions, and their growth conditions are very different. Although they belong to the same family, they are of different species. Through testing, the medium formula disclosed in the prior art is not suitable for Proliferation of Tissue Culture Nodes of Aristolochia Nonggang

Method used

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  • Proliferation culture medium for aristolochia longgonensis tissue culture and application of proliferation culture medium
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  • Proliferation culture medium for aristolochia longgonensis tissue culture and application of proliferation culture medium

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Embodiment 1

[0012] Prepare culture medium, wherein the components of each liter of the culture medium and the content of each component are as follows:

[0013] 1. Macroelement: KNO 3 1900mg / L; NH 4 NO 3 1650mg / L; MgSO 4 ·7H 2 O370mg / L; CaCl 2 440mg / L; KH 2 PO 4 340mg / L.

[0014] 2. Trace elements: KI 0.83mg / L; H 3 BO 3 6.2mg / L; MnSO 4 4H 2 O 22.3mg / L; ZnSO 4 ·7H 2 O 8.6mg / L; Na 2 MoO 4 2H 2 O 0.25mg / L; CuSO 4 ·5H 2 O 0.025mg / L; CoCl 2 ·6H 2 O0.025mg / L.

[0015] 3. Phytohormone: 6-BA0.5mg / L-1.0mg / L.

[0016] 4. Others: sucrose 30g / L; agar 4g / L; water; pH5.8.

[0017] 5. Culture medium preparation: add according to the content of each component, in the order of sucrose→macroelements→trace elements→phytohormones→mixture of boiled agar and water→add water to volume→adjust pH to 5.8→pack into jam bottles Neutralize and seal well → Autoclave sterilization at 121°C for 20 minutes.

[0018] 6. Inoculation method: Take the Nonggang Aristolochia bottle seedlings that ha...

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Abstract

The invention discloses a proliferation culture medium for aristolochia longgonensis tissue culture and an application of the proliferation culture medium. The sectional proliferation culture of artistolochia longgonensis can be carried out through the improved MS culture medium plus plant hormones with certain concentration. Due to the adoption of the proliferation culture medium, the problem that the formulas of other section proliferation culture media are not suitable for the sectional proliferation culture of the aristolochia longgonensis can be solved, an optimal proliferation effect can be obtained by utilizing the improved MS culture medium plus the 6-BA cytokinin with a reasonable concentration, and the proliferation efficiency can be increased.

Description

technical field [0001] The invention belongs to the field of agricultural planting, and in particular relates to a proliferation medium for tissue culture of Aristolochia nongang and its application. Background technique [0002] Aristolochia Nonggang is a plant of the genus Aristolochia of the Aristolochiaceae of the order Pepper, scientific name: Aristolochia longgonensis C.F.Liang, also known as Nonggang Tongcheng Tiger. In 1979, it was discovered by the Guangxi Nonggang Comprehensive Investigation Team in the Guangxi Nonggang National Nature Reserve that it is a vine medicinal plant under the karst forest on the northern margin of the tropics in Guangxi. Similar, it has a good analgesic effect, and is used in the treatment of pain, snakebite, high fever and other diseases among the people. The wild Aristolochia Nonggang in China is mostly distributed in the Nonggang National Nature Reserve in Guangxi. [0003] "Tissue Culture and Rapid Propagation of Aristolochia akebi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 廖韦卫谢君锋唐君海罗晟昇李根蒋亚琴俸青莫周美何洪良陆祖正唐利球符策秦昌鲜
Owner 广西橡胶研究所
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